Chemistry and antioxidant potential of Tinospora cordifolia extracts and its bioactive compound(s)
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Date
2022
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Punjab Agricultural University
Abstract
The present investigation deals with chemistry and antioxidant potential of Tinospora cordifolia extracts and its bioactive compound(s). The mineral analysis of the stem powdered showed the presence of potassium in the highest concentration whereas cadmium in the lowest concentration. The methanol extract of stem was prepared using Soxhlet apparatus and partitioned into polar and non-polar fractions using dichloromethane, chloroform and hexane as solvent. Qualitative analysis of phytochemicals affirmed the presence of phenols, flavonoids, saponins, alkaloids, tannins, terpenoids, steroids and cardiac glycosides in methanol extract. The total phenolic, flavonoid and alkaloid content of methanol extract was found to be 2.90±0.36mg GAE g-1, 3.79±0.48mg QE g-1 and 0.09379±0.45 mg B g-1, respectively. The major compound berberine was isolated from the chloroform fraction of T. cordifolia using column chromatography and the structure of compound was elucidated on the basis of spectroscopy analysis. Antioxidant potential was carried out using five different in vitro models involving 2, 2-diphenyl-1-picrylhydrazyl (DPPH) , hydroxyl (OH), nitric oxide (NO), superoxide (O2) radicals scavenging methods and ferric reducing antioxidant power assay (FRAP) at various concentrations (0.02-0.1 mg ml-1). The antioxidant activity increased with increase in concentration. Methanol extract was found to be most active as compared to other fractions. The antioxidant activity of methanol extract was comparable of berberine. Hence, methanol extract and berberine showed good potential as a source of natural antioxidants.
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Poonamdeep Kaur (2022). Chemistry and antioxidant potential of Tinospora cordifolia extracts and its bioactive compound(s) (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.