Inheritance and Mapping of Ascochyta Blight Resistance Derived from Chickpea (Cicer Arietinum L.) Cultivar ‘PBG 7’
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Date
2023
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Punjab Agricultural University
Abstract
In the present study, a total of 208 RILs derived from cross HC 5 ᵡ PBG 7 were evaluated for inheritance studies and mapping of Ascochyta blight resistance during rabi season 2021-22 in Experimental Area of Pulses Section, Ascochyta blight nursery and Molecular Biology Laboratories, Punjab Agricultural University, Ludhiana. The plant material was planted in α-lattice plot design with two replications for assessment of important agronomic traits. The RIL population segregated in the ratio 3 (Resistant): 13 (Susceptible) for Ascochyta blight disease and indicated that the Ascochyta blight resistance was governed by two genes and there was epistatic interaction between two genes, with dominant allele of a gene (suppressor gene) at one locus suppressing the action of both dominant and recessive alleles of second gene at a completely different locus. For mapping of AB resistance, sequencing based Bulk Segregant Analysis approach was used and four potential QTLs (3 on chromosome no. 4 and 1 on chromosome no. 6) were identified. For validation of identified QTLs, 47 SNP based KASP markers were designed and validated on parents and bulks. Out of 47 KASP markers, 15 showed polymorphism (9 markers for chromosome no. 4 and 6 markers for chromosome no. 6). Using the information of position of these markers on linkage groups, 2 genomic regions (1.75 Mb on chromosome no. 4 and 0.212 Mb region on chromosome no. 6) associated with AB disease response were tagged. Upon statistical analysis of phenotypic data, it was observed that a total of 96 RILs and 98 RILs were significantly better than both the parents for number of pods per plant and total seed yield respectively.
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Manisha Rani (2023). Inheritance and Mapping of Ascochyta Blight Resistance Derived from Chickpea (Cicer Arietinum L.) Cultivar ‘PBG 7’ (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.