Influence of antibiotic substitutes on bacterial load and semen quality of buffalo bulls
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Date
2024-05
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Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar
Abstract
The aim of the present study was to evaluate the bacterial load and semen quality of buffalo
bull semen in fresh and cryopreserved semen samples by using extenders supplemented with
antibiotics, without antibiotics and substitutes of antibiotics. For this study, ejaculates were collected
from sixteen Murrah bulls and two experiments were performed. The second experiment was further
divided into two independent experiments (1) and (2) respectively. For the estimation of microbial load
80 ejaculates were collected through AV method for experiment 1 and semen quality was assessed in
16 samples, whereas 5 and 16 ejaculates respectively for experiment (1) and (2) for estimation of
microbial load and semen quality. For experiment 1, each ejaculate was divided into two aliquots: 1)
control (NAB), 2) extender supplemented with antibiotics (AB). For experiment (1) of second
experiment, semen samples were diluted in extenders supplemented with KA (0.02, 0.04, 0.06, 0.08
and 0.1g/L), AB and control (NAB), for experiment (2), extenders supplemented with epsilon polylysine (0.64 and 1.28g/L), AB and control (NAB) and cryopreserved. After post-thaw, the
following parameters were studied; thermal resistance of sperm by incubation test, sperm motility and
kinetics by (CASA), acrosome integrity, mitochondrial membrane potential (MMP) and mitochondrial
superoxide levels through flow cytometry. The results of the present study showing a non-significant
difference in bacterial load (CFU/mL) and a non-significant effect on sperm motility and kinetic
parameters, plasma membrane integrity, HMMP and increased superoxide levels in AB and NAB
groups. There was no significant difference in endotoxin levels estimated by kit in both the groups. The
results of different treatment groups of Kojic Acid in comparision to AB and NAB showed non significant difference in bacterial load and sperm quality parameters. The results of different treatment
groups of epsilon-polylysin showed non-significant decrease in bacterial load whereas quality
parameters were improved significantly. In conclusion, the microbial load was controlled in semen
extenders without the addition of antibiotics and sperm quality parameters parameters were equivalent
in group AB and NAB. Addition of Kojic acid in semen extender did not show any antibacterial effect
whereas epsilon-polylysin showed antibacterial effect and improved semen quality.