STUDIES ON VIRUS DISEASE OF CAPSICUM (Capsicum annuum L.)
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Date
2021-12-22
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University of Horticultural Sciences Bagalkot. (College of Horticulture, Bagalkot).
Abstract
A study on virus disease of capsicum was undertaken at Horticultural Research and
Extension Centre, Devihosur, Haveri during 2019-20. The roving survey results revealed that
the capsicum virus disease was prevalent in all the surveyed districts of Karnataka. The
average per cent disease incidence in overall surveyed districts was 70.46%. Among five
districts surveyed, highest disease incidence was recorded in Haveri (89%), followed by
Belagavi (84%), Chikkaballapur (76%) and Kolar (60%) whereas the least disease incidence
was recorded in Bengaluru rural (43.33%). Similarly, among the 10 taluks, highest disease
incidence of 92% was observed in Byadgi followed by Ramdurga (86.66%), whereas the least
incidence of 43.33% was recorded in Nelamangala. Similarly, among the 22 villages, highest
incidence of 100% was recorded in Bannahatti village of Byadgi, whereas the least incidence
of 20% was recorded in Tadshigatta village of Nelamangala.
Upon amplification by PCR, 531 bp and 1500 bp size amplicon was amplified in
diseased sample of capsicum and also in diseased sample of chilli (positive control) and no
amplification was obtained in healthy sample of capsicum (negative control). No
amplification was obtained with tospovirus, PMMoV, CMV, TMV, TEV, ToMV. Thus, the
investigation focused on molecular identification of virus associated with disease of capsicum
by employing PCR techniques convincingly revealed that ChiVMV and ChiLCV association
with virus disease of capsicum was determined.
The host range studies revealed that the potyvirus (ChiVMV) isolate infected
members of family Solanaceae only. Among the tested plant species, it has infected Datura
metel, Datura stromonium, Nicotiana tabacum L. var. White burly and Nicotiana glutinosa in
addition with chilli and capsicum (bell pepper). No symptoms were observed on sap
inoculated plants of cucumber, cowpea and beans.
Among the twenty genotypes screened, none of them were found to be immune, two
genotypes viz., Diana and Indra were found resistant and Yalavigi selection was found
moderately susceptible. However, twelve genotypes viz., DCA-246, DCA-308, DCA-311,
DCA-315, DCA-329, DCA-346, DCA-347, DCA-355, Priyanka, Asha F1, Nemalite, and
Indus-11 were found to be susceptible. Five genotypes viz., DCA-310, Tarihal selection and
Arka Mohini, Arka Gourav and Arka Basant were found to be highly susceptible.