Serological and Molecular Studies on Brucellosis and Q-fever in Goats of Haryana
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Date
2024-04
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Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar
Abstract
Brucellosis and Q-fever are two important zoonotic bacterial diseases that cause reproductive
problems in goats, including mid to late-term abortions and has a significant impact on animal
production and public health. Despite this, there have been very few studies on the prevalence of
brucellosis and Q-fever in goats in Haryana. The present study was done to determine the prevalence of
Q-fever and brucellosis in Haryana. A total of 400 sera sample was collected from serum bank of
Department of Veterinary Microbiology, LUVAS, Hisar and were analyzed using RBPT and iELISA
for brucellosis and iELISA for Q-Fever. Moreover, 10 clinical samples with serum were collected from
mid-term to late-term aborted goats in Haryana and subjected to PCR using bcsp31 and IS711 primers
for Brucella Spp., and IS1111 for Coxiella burnetii.
The overall seroprevalence was estimated as 13.75 percent by RBPT and 16.5 percent by
iELISA for brucellosis and 18.25 percent by iELISA for Q-fever. It was observed that 4.5 percent
samples were serologically positive for both Brucellosis and Q-fever by iELISA. The seropositivity of
brucellosis and Q-fever in mid to late-term aborted goats of Haryana was observed as 20 percent by
RBPT and iELISA for brucellosis and none of the samples were found positiveby iELISA for Q-fever.
By using conventional PCR, 20% (2/10) clinical samples were found positive for B. melitensis and
10% (1/10) were positive for C. burnetii. Subsequently, it suggests that serological and molecular tests
are suitable for diagnosing brucellosis and Q-fever in goats. Furthermore, vaccination programmes,
public awareness and campaign programmes should be done to teach farmers regarding hygiene,
sanitation and to stop spreading of disease.