Molecular characterization of segregating f6 generation of okra (Abelmoschus esculentus (L.) moench)
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Date
2023-02-23
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Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Abstract
Okra is a nutrient-rich important crop with commercial value in tropical and subtropical regions of the world. In present investigation, eighteen okra genotypes were used and performances of okra genotypes on various yield attributing characters were assessed. Field screening of eighteen okra genotypes for various yield attributing was done. The genotype PBNLF-122 was observed as high yielding genotype that shows better performances viz.,number of branches per plant (1.62), number of nodes per plant(11.65), number of fruit per plant (11.34), fruit weight(12.10) and fruit yield per plant(157.85) whereas genotype PBNLF-116 gave the least performances.
Three molecular markers systems, RAPD, ISSR, and SSR, were used to identify genetic relationship among okra genotype. Based on the number of bands and the nature of amplification, 12 RAPD primers, 3 ISSR primers and 6 SSR primers were selected for the study Two RAPD primer OPY-18, OPG-08 showed 100% polymorphism OPY-18 was found to be the most informative marker,withaPICvalueof0.81. UBC 840 were found to be the most informativemarker,withaPICvalueof0.22 and 83.33%. Out of five SSR primersOYVMVwasobservedtobe the most informative with the PIC value 0.77. Genetic similarity was estimated using clubbed data of RAPD, ISSR, and SSR using Jaccard’s coefficient which is ranged from 0.61 (PBNLF-124 and PBNLF-122) to 0.87 (PBNLF-139 and PBNLF-109), with 0.74 being theaverage.The genetic distances ranged from 0.13 to 0.41 with an average 0.27 among these eighteen genotypes of okra. This confirmed that the ultimate genetic distance was maximum in two i.e. 0.41 between PBNLF-122 and PBNLF-104 and second PBNLF-123 and PBNLF-114; The outcome of the field analysisand molecular analysis can be used for hybridization programs and crop improvement program of segregating okra genotypes.