STABILITY ANALYSIS AND MOLECULAR PROFILING OF DESI CHICKPEA (Cicer arietinum L.)
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Date
2022
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DRPCAU, PUSA
Abstract
The aim of the current study "Stability analysis and molecular profiling of Desi Chickpea (Cicer arietinum L.)" genotypes in different environments was to determine the most stable genotype under various environmental conditions and to estimate stability parameters for seed yield and its significant components. To determine the stability of genotypes for various traits, thirty genotypes were sown on three distinct sowing dates using a Randomized Block Design with three replications. Observations were recorded for fourteen traits viz., plant height (cm), days to 50% flowering, days to maturity, number of primary branches, number of secondary branches, number of pods per plant, number of nodules per plant, nodule fresh weight (g), nodule dry weight (g), root length (cm), root volume (ml), 100 seed weight (g), harvest index (%) and grain yield per plant (g). The Eberhart and Russell (1966) model of statistical analysis was used to examine the data.
Pooled analysis of variance for different studied characters between the genotypes shown significant variation for the most of traits indicated the presence of genotypic variation in studied desi chickpea genotypes. The high environmental variance for all attributes studied suggested that environmental conditions were varia-ble. The components (linear) of G x E interaction were found highly significant for most yield attributing traits indicating that these traits were unstable and shown fluctuations in their expression with environmental change.
Total three genotypes out 30 genotypes noticed yield stability in favorable/unfavorable environments. In favorable environment one genotype ICCV-18-1664 with below average stability and in unfavorable environment two genotypes DC-17-1111 and DC-17-1115 were sable with above average stability. None of the genotype exhibited average and absolute stability for studies characters for all envi-ronments.
Nei’s Genetic Diversity (h) and Shannon Index (I) within the population of 30 genotypes yielded the value of 0.318 and 0.446 respectively, suggesting that the population of 30 chickpea genotypes is moderately diverse. Dendrogram analysis on the base of dissimilarity index clustered the 30 genotypes into 4 main cluster with cluster-I having the highest numbers of genotypes with eleven genotypes followed by cluster-II with nine genotypes and cluster-III with seven genotypes, cluster-IV with three genotypes. The genetic Structure of 30 genotypes at K=4 (based on 15 SSR primers with 55 amplified loci) revealed that the 30 desi chickpea genotypes are the admixture of four ancestral components present in different combinations in 30 genotypes.