Pyramiding of brown plant hopper resistance genes Bph34 and Bph33 in rice
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Date
2022
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Punjab Agricultural University, Ludhiana
Abstract
The present study was conducted to pyramid brown plant hopper resistance genes Bph34 and
Bph33 in rice. Brown planthopper (BPH) (Nilaparvata lugens Stal.) is the most devastating
insect pest of rice, sucking the phloem sap of plants and causing hopper burn symptoms and
massive output losses every year. The present study utilized phenotypic and genotypic
evaluation of F1 and BC1F1 mapping populations derived from the complex cross for BPH
disease resistance. A total of 50 F1 plants and 120 BC1F1 progenies were analyzed through
foreground selection using linked polymorphic SSRs Bph34 and Bph33 gene. Out of 50 F1
plants assessed, 2 accessions were found to be homozygous for Bph33 gene, 13 accessions
were found to be homozygous for Bph34, 7 accessions were heterozygous for both Bph33 and
Bph34 genes, while 28 accessions were devoid of both Bph33 and Bph34 genes. Foreground
selection of the BC1F1 population was carried to ascertain presence of homozygous alleles for
both genes. From a total of 120 BC1F1 individuals, 21 plants were found to be heterozygous
for target trait loci. 36 individuals were found to have only Bph33 gene while 34 were
observed to possess only Bph34 gene. 14 progenies were found to be positive for both Bph33
and Bph34 genes, however, 8 individuals did not carry any of target genes. The results were
confirmed through phenotypic assessment as well. Phenotypic screening against BPH
resistance was done and these lines showed high level of BPH resistance. Background
selection was carried using 377 SSR markers of rice among four parents and 74 SSRs markers
were found to be polymorphic. The positive plants were found to be agronomically superior
can be further subjected to background selection as well as in breeding programs for varietal
release purpose.
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Citation
Thakur, Arti (2022). Pyramiding of brown plant hopper resistance genes Bph34 and Bph33 in rice (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.