PHYSIOLOGICAL AND GENETIC DIVERSITY ANALYSIS OF SELECTED ADVANCED BREEDING LINES OF CLUSTER BEAN [Cyamopsis tetragonoloba (L.) Taub] FOR DROUGHT TOLERANCE
Loading...
Date
2017-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru).
Abstract
Cluster bean [Cyamopsis tetragonoloba (L.) Taub] is an important under exploited
leguminous vegetable crop adapted to arid drought conditions. Even though the crop is drought
tolerant in nature, the yield will decrease under severe moisture stress condition hence the study
has been conducted to identify the better advanced breeding lines which will suit to water deficit
conditions. Nineteen advanced breeding lines including the check variety Pusa Navabahar were
raised in pots and screened for relative water content (RWC), which is the most appropriate
estimate of plant water status in terms of physiological consequence of cellular water deficit 35
DAS under drought, 7 lines including check were selected for further water stress studies. Stress
was imposed by withholding water after 35 DAS except for well watered plants. Physiological
parameters such as RWC, SPAD chlorophyll meter, total leaf area, specific leaf area, root traits,
leaf area ratio and total dry matter were recorded. Advanced breeding lines like COHBCB 15S2,
COHBCB 30S1, COHBCB TL-2 which performed better under water stress condition. Further
for evaluating genetic diversity, 22 microsatellite markers were used among 19 lines. A
dendrogram was constructed based on unweighed pair group method of arithmetic means
(UPGMA) using data derived from 22 SSRs grouped the 19 cluster bean advanced breeding lines
into two main clusters with 0.62 similarity. The range of dissimilarity among the cluster bean
advanced breeding lines varied from 0.62 to 1.00.