Detection and transmission of phytoplasma/virus(es) infecting brinjal (Solanum melongena L.) in Punjab
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Phytoplasma/virus disease complex is a major obstruction against brinjal production. In
present study, survey conducted in major brinjal growing areas of Punjab and observed that
phytoplasma/virus disease complex was present in all surveyed areas having disease
incidence ranging from 8-21 per cent causing different type of symptoms viz. yellow mosaic,
mottling, wavy margin, puckering, leafroll, little leaf, giant calyx, big bud, phyllody,
virescence, stunting and withes’ broom. Under Punjab conditons association of five RNA
viruses viz. PepMoV, PLRV, PVX, PVYn, PVYo/c, ssDNA virus (Begomovirus) and
phytoplasma (Aster yellows phytoplasma) were revealed with this disease complex. The
begomoviruses associated with this disease complex were identified as Tomato leaf curl New
Delhi virus and Tomato leaf curl Palampur virus. Under transmission studies whitefly was
confirmed as the vector of begomoviruses. Histopathological studies of leaf lamella (showing
little leaf), flower petal (showing phyllody) and giant calyx divulged many ultrastructural
changes in dermal, ground and vascular tissues. In search of host plant resistance 135
cultivated genotypes and six wild relative species of brinjal were screened against
phytoplasma/virus disease complex, under open field conditions. Out of these 24 cultivated
genotypes and three wild species were screened against begomovirus, under artificial
screening conditions. Screening results revealed that brinjal genotype P-67 and two wild
species viz. Solanum sisymbrifolium and S. torvum were showing resistance against
begomovirus however, in PCR assay using PALIc1960/PARIv722 primers all 24 genotypes
were showing positive reaction for presence of begomovirus. But S. sisymbrifolium and S.
torvum were showing negative reaction in PCR.
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Citation
Sen, Bheem (2021). Detection and transmission of phytoplasma/virus(es) infecting brinjal (Solanum melongena L.) in Punjab (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.