Evaluation of boar semen stored between 16-20° C using Lactose-egg yolk, Glucose-sodium citrate dihydrate and Modena diluents
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Date
2021
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Abstract
This study was carried out to compare the efficacy of certain liquid semen extenders
for boar semen preservation.Semen ejaculates (n=30) from three boars (one
ejaculate/boar/week) collected using dummy sow were extended (1:4) in lactosesodium
salt of EDTA-Egg yolk (LSEEY), glucose sodium salt of EDTA-potassium
sodium tartrate-sodium citrate dihydrate (GEPS), Modena (MOD). The extended
semen was kept at 17°C upto 120 h in a BOD incubator. The semen was assessed for
various sperm characteristics and lipid peroxidation at 0, 24, 48, 72, 96 and 120 h of
preservation. The results revealed significantly (P<0.05) higher percentage of sperm
motility, viability, plasma membrane integrity and acrosome integrity in semen
extended with MOD than in GEPS and LSEEY at different preservation times. The
mean percentage of in vitro capacitation/acrosome reacted spermatozoa was
significantly (P<0.05) higher in MOD as compared to GEPS and LSEEY at 96 h and
120 h of storage period. In all the extenders, the mean percentage of most sperm
attributes decreased (P<0.05) progressively as the storage period increased.
Correspondingly, the MDA levels were lower (P<0.05) in MOD than in their
contemporary extenders at all hours of preservation. In conclusion, MOD maintained
the sperm parameters and reduced oxidative stress for liquid preservation of boar
semen in extended period of storage at 16-20°C.