EVALUATION AND CHARACTERIZATION OF PUTATIVE MUTANT POPULATIONS IN MANGO (Mangifera indica L.)
Loading...
Date
2018-10
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, GKVK POST, BANGALURU)
Abstract
The present investigation on “Evaluation and characterization of putative mutant populations in mango (Mangifera indica L.)” was carried out in the Division of Fruit crops, ICAR-IIHR, Hessaraghatta, Bengaluru, 2017-2018. Two different mutagenic populations have been used for the study which includes EMS and colchicine treated populations. The morphological, physiochemical and molecular variations reveal that reduced plant height, longer leaf length, leaf width, shorter internodal length and petiole length were observed in both the populations generated from different concentrations of Ethyl Methane Sulphonate (EMS) and colchicine. The total phenol content in leaf was maximum in 0.8 per cent EMS and 1 per cent colchicine treated mutants, which was correlated with dwarf plants. The mean stomata size was observed to be bigger in plants treated with EMS and colchicine in comparison to the control. Biochemical parameters such as total chlorophyll content got reduced with high doses of mutagen and the increase in activity of enzymes like peroxidase and poly phenol oxidase was also noticed. The negative correlation of plant height with the phytohormones like ABA content and positive correlation of GA was observed. The molecular characterization was employed using SSR markers to confirm the variability within the mutant progenies. The Polymorphic Information Content (PIC) values ranges from 0.6644 to 0.7238 and 0.6507 to 0.7106 in mutant progenies of mango cvs. Arka Puneet and Alphonso respectively and found to be informative primers. So, from the present study it can be concluded that both EMS and colchicine (0.8 to 1.0 per cent) can be used to create variability and broaden the genetic base in mango.