Formation and characterization of Apium graveolens L. nanoemulsion and it's herbicidal potential
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
Essential oil based bioherbicides are topic of interest for the new era agriculture because of
their sustainable use in the control of weeds. The present study aims to formulate
nanoemulsion of celery (Apium graveolens L.) essential oil (EO) and evaluate its herbicidal
activity against Phalaris minor Retz. and Avena ludoviciana Durieu. Chemical composition
of EO from seeds A graveolens showed presence of thirty-seven compounds amongst which
limonene (76.95%) was the major compound. Nanoemulsions were fabricated by high
frequency ultrasonic method and phase diagram was mapped and optimum O/W formulations
were selected. The physicochemical properties recorded for nanoemulsion indicated their
O/W and isotropic nature. Transmission electron microscopy (TEM) and Zavg indicated
spherical nature of droplet with average size to be > 21 nm. The prepared formulations were
stable to storage and centrifugation. Formulated emulsion and nanoemulsions having 0.05%
and 0.1% A. graveolens EO completely inhibited the germination of P. minor and A.
ludoviciana by adversely affecting the physiological processes like seed imbibition and
membrane leakage mediated cellular damage without having adverse effect on Triticum
aestivum.
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Gurinder Singh (2021). Formation and characterization of Apium graveolens L. nanoemulsion and it's herbicidal potential (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.