Introgression of yellow vein mosaic disease resistance in okra
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation was undertaken with the objectives to screen okra wild
relatives, study inheritance of yellow vein mosaic disease, identification of marker(s) linked to
YVMD resistance and to evaluate introgressed lines for economic traits. Two year consecutive
artificial YVMD screening of sixteen entries of seven wild species and two varieties of cultivated
okra (Punjab Padmini and Pusa Sawani) revealed Abelmoschus angulosus accessions IC-470751
and IC-203833 and Hibiscus acutetus accessions SBT-12557 and SBT-12592 highly resistant to
YVMD. A. moschatus accession IC-140986, A. caillie and A. manihot (PAUAcc-1) exhibited
moderately resistant reaction at 90 DAI. Whereas, A. moschatus accession IC-141056, A. manihot
accession EC-329394, A. tuberculatus and cultivated okra variety Punjab Padmini manifested
susceptible and cultivated okra variety Pusa Sawani highly susceptible reaction to YVMD up to 90
DAI. Deng Begomovirus specific universal primer was used to confirm the YVMD resistance.
The YVMD resistant plants of A. angulosus (IC-203833) and A. manihot PAUAcc-1 were used as
pollen parent for the development of F1, BC1F1, BC1F2 and BCIF3 population using Punjab
Padmini as the female parent. Two recessive genes were found to govern resistance to YVMD as
revealed by the appearance of susceptible and resistant plants in the ratio of 9:7 in the BC1F2
generation. Out of 200 SSR markers, 31.5% were found polymorphic between the two parents
Punjab Padmini and A. manihot (PAUAcc-1). Four primer pairs viz. Okra 032, Okra 049, Okra
129 and Okra 270 differentiated the two contrasting YVMD resistant and susceptible bulks in bulk
segregant analysis. A set of 137 BC1F3 progenies of Punjab Padmini x A. manihot (PAUAcc-1)
were evaluated along with five checks (Punjab Padmini, Pusa Sawani, PORL, POL-44 and Punjab
Suhawani) for yield and its contributing traits. Fifty seven progenies were found to be free from
YVMD up to 90 DAI. Seventy four progenies having marketable yield in the range of 112.5 to
189.6 g/plant were observed to be significantly superior to standard check Punjab Suhawani
(111.9 g/plant). Progeny PAUMIOL-56 was at top for yield (189.62 g/plant) followed by
PAUMIOL-36, PAUMIOL-138, PAUMIOL-105 and PAUMIOL-142 and had no YVMD
incidence upto 90 DAI. Similarly, 88 BC1F3 progenies of Punjab Padmini x A. angulosus (IC203833)
were accessed along with seven checks (Punjab Padmini, Punjab Suhawani, PORL, POL44,
POL-78 and Pusa Sawani) for economic traits. Six progenies recorded marketable yield in
range of 108.2 to 130.2 g/plant significantly higher than standard check Punjab Suhawani (97.9
g/plant). Forty one progenies were free from YVMD up to 90 DAI. Progeny PAUAIOL-324
yielded maximum (130.2 g/plant) followed by progeny PAUAIOL-251, PAUAIOL-301,
PAUAIOL-256 and PAUAIOL-283 and had no YVMD incidence up to 90 DAI.
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Citation
Gagandeep Singh (2021). Introgression of yellow vein mosaic disease resistance in okra (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.