STANDARDIZATION OF PROTOCOL FOR EXTRACTION OF ANTHOCYANIN FROM ROSELLE (Hibiscus sabdariffa L.) CALYCES FOR EDIBLE COLOUR
Loading...
Date
2015-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru).
Abstract
Roselle (Hibiscus sabdariffa L.) is a multi-use plant whose calyces are potentially good source of antioxidant agents like anthocyanins, phenols and ascorbic acid. Studies were carried out to extract anthocyanin pigments from roselle calyces using different solvents, fermentation, enzyme and hot water methods and their storage stability and cost economics of extracted pigment at Department of Postharvest Technology, COH, Bengaluru during 2014-15.
Studies revealed that, significantly highest anthocyanin content (1638.17 mg 100 ml-1), total antioxidants (101.05 mg 100 ml-1), TSS (28.40 °B), total phenols (12.84 mg ml-1) and titratable acidity (10.83%) was obtained in the treatment ethanol acidified with 1.5N HCl. The least pH (1.26) and water activity (0.80) was recorded in same treatment during initial T0 period. At 90 days after storage (DAS) under ambient and refrigerated conditions, ethanol acidified with 1.5N HCl recorded highest anthocyanin content of 802.62 and 1091.92 mg 100 ml-1, total antioxidants of 32.06 mg 100 ml-1 and 43.02 mg 100 ml-1, total phenols 4.29 and 5.56 mg ml-1, titratable acidity 9.73 and 10.18 per cent and TSS 29.30 and 28.93 °B respectively. Same treatment recorded least pH 1.62 and 1.59 and water activity 0.78 and 0.78 at ambient and refrigerated storage conditions respectively.
The B:C ratio (24.88:1) was highest in the treatment fermentation of calyces, but retention of anthocyanin content was lower after storage for 90 DAS. Overall present investigation revealed that, treatment ethanol acidified with 1.5N HCl was found to be best with highest anthocyanin retention and total antioxidants with better B:C ratio (8.81).