“DEVELOPMENT AND EVALUATION OF RIL AND BIL POPULATIONS FROM A BI-PARENTAL CROSS BETWEEN Citrullus lanatus var. citroides AND Citrullus lanatus (cv. Arka Manik) FOR MAPPING YIELD, QUALITY AND DISEASE RESISTANCE TRAITS”
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Date
2017-12
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University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru).
Abstract
The study included development and evaluation of sub-specific mapping populations in watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] for mapping of yield, quality traits and resistance to fusarium wilt. The mapping population was developed through single seed descent method. Subsequently, a total of 141 Backcross Inbred Lines (BIL’s) and 154 Recombinant Inbred Lines (RIL’s) of mapping populations were screened artificially using two isolates (KNL and N) of Fon. Thirty three BIL and 18 RIL genotypes showed less than 33 per cent mortality after 15 days of inoculation in isolate-KNL. Whereas, for isolate-N twenty five BIL and 32 genotypes of RIL showed less than 33 per cent mortality after 15 days of inoculation. Twenty backcross inbred lines and nineteen recombinant inbred lines possessed combined resistance to both isolates (Isolate-KNL and Isolate-N) for fusarium wilt resistance. A total of 147 markers from BIL population and 156 markers in RIL population were used to develop linkage map. The developed map comprised of 11 chromosomes covering a distance of 2518 cM and 2160 cM having mean marker interval of 17.13 cM and 13.84 cM in BIL and RIL population respectively. In RIL population for isolate-KNL, one major QTL was detected on chromosome 1 (Kfon1.1). For N isolate, two major QTLs were detected on chromosome 10 (Nfon10.1 and Nfon10.2) in BIL population. One major QTL was detected for fruit weight in BIL population on chromosome 8 (Fwt8.1). One major QTL (TSS2.1) in BIL population and one minor QTL in RIL population were detected on chromosome 2 (TSS2.2). The resistant lines of the populations can be used as pre bred lines for resistance breeding and the course map developed, can be refined with increase in marker number and population size.