MUTAGENIC STUDIES IN MANGO (Mangifera indica L.)
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Date
2016-08
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University of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, UHS CAMPUS, GKVK POST, BENGALURU)
Abstract
Mango is one of the most important fruit crops valued for its rich nutrient content mainly vitamin A. The present investigation on “Mutagenic studies in mango (Mangifera indica L.)” was carried out in the Division of Fruit crops, ICAR-IIHR, Hessaraghatta, Bengaluru, 2015-2016. The experiment on mutation was carried out using the chemical mutagen Ethyl Methane Sulphonate (EMS), and in another experiment growth regulator GA3 has been used to increase the efficacy of EMS. The hybrid Arka Puneet was used with the objective of inducing variability among the mutant progenies. The observations were recorded at different intervals of time after sowing in polyhouse and after transplanting in field (30, 60 & 90 DAS and DAP respectively). Mutant progenies from different concentrations of EMS were evaluated for morphological, physiological and molecular characteristics. Morphological changes were observed both under polyhouse and field conditions. The total phenol content in leaf was maximum in 0.6 per cent EMS treated mutants, where more number of dwarf plants were observed. The molecular characterization was employed using SSR markers to confirm the variability within the mutant progenies with the Polymorphic Information Content (PIC) values ranging from 0.67 to 0.91. The stones of ‘Kishen Bhog’ cultivar were treated with different concentrations of GA3 followed by EMS (0.4%) showed that GA3 increases the efficacy of EMS. From the present study it was concluded that EMS can be used as a mutagen to create variability in mango and also the efficacy of the mutagen can be enhanced by using GA3.