MOLECULAR CHARACTERIZATION AND VARIABILITY OF Alternaria burnsii (UPPAL, PATEL & KAMAT) CAUSING BLIGHT OF CUMIN
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Date
2019
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DEPARTMENT OF PLANT PATHOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND
Abstract
Cumin (Cuminum cyminum L.) popularly known as Jeera or Jiroo is the most
important spice crop in India. Gujarat is second largest producer next to Rajasthan
with 50-55% of total production of India. Cumin crop suffers with blight disease
caused by Alternaria burnsii. Looking to the seriousness of the disease, investigations
on certain aspects of the management were carried out and reported here as under:
The isolation was made from infected seeds, branches, stems and umbels of
cumin plants showing typical blight symptoms were collected from Ahmedabad,
Surendranagar, Banaskantha, Patan and Morbi (isolates designated as Ab-1 to Ab-15)
areas of Gujarat. These revealed the association of A. burnsii. The isolated fungus, A.
burnsii satisfied Koch’s postulate on cumin plant. The identity of Alternaria burnsii
was confirmed by DNA sequencing done at the Department of Agricultural
Biotechnology, BACA, AAU, Anand and the rDNA homology searches were
performed using the BLAST program through the internet server at the National
Center for Biotechnology Information (National Institutes of Health, Bethesda, USA).
Sequences and accession numbers for compared isolates were retrived from the
GenBank database. The sequenced rDNA region of Alternaria burnsii (Accession no:
MK758067, MK764688, MK764690, MK764695, MK764710, MK764711,
MK764712, MK764716, MK770618, MK770619).