METAGENOMIC ANALYSIS OF CAMEL RUMEN MICROBIAL DIVERSITY FOR MINING GLYCOSIDE HYDROLASES
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Date
2019
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Department of Animal Genetics & Breeding College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand
Abstract
India possesses 9 breeds of camel, having a population of 4 million numbers. Presence of diverse groups of microorganism, especially anaerobic and facultative anaerobic bacteria, in the forestomach of camels have a crucial role in their physiological development and proper feed utilization. Aim of the present study was to reveal the bacterial diversity in different fractions of camel rumen liquor at different time intervals in response to feeding of different fiber diet (Guar, Bajra, Maize and Jowar). Metagenomic DNA was isolated from rumen samples of fourteen adult camels of Bikaneri and Kachchhi breeds followed by amplicon sequencing of V3-V4 hypervariable region of prokaryotic 16S rRNA gene in Illumina MiSeq platform. A total of 20.17 million resultant raw reads were clustered into 7,906 Operational Taxonomic Units (OTU) at 97% sequence similarity and taxonomically classified into 36 phyla and 415 genera, by using Greengene reference database, through QIIME pipeline. Phyla Bacteroidetes, Firmicutes, Proteobacteria, Fibrobacter and Verrucomicrobia were significantly dominant in all feed groups (p < 0.05).