Molecular mapping and interspecific hybridization for hull-less seed trait in pumpkin for value addition in food processing industry
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Date
2020
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Punjab Agricultural University, Ludhiana-
Abstract
The present investigation entitled ‘Molecular mapping and interspecific hybridization for
hull-less seed trait in pumpkin for value addition in food processing industry’ was conducted
in the Department of Vegetable Science, Punjab Agricultural University, Ludhiana from 2016
to 2020. Segregating populations were developed using hulled and hull-less genotypes of
Cucurbita pepo for understanding the genetics and molecular mapping of hull-less seed trait.
Chi-square analysis of segregating populations revealed that single recessive gene is
responsible for controlling this trait. The sequence based bulked segregant analysis of two
extreme bulks (hulled and hull-less) of cross HP111 × HLP36 along with their parental lines
identified one QTL on chromosome 12 (Cp4.1LG12) between 1891261 to 3863267 bp
covering 1.97 Mb genomic region with 396 number of SNPs for hull-less seed trait. In
interspecific hybridization, total 916 crosses between C. pepo (HLP36, HLP44, HLP53,
HLP72) and C. moschata (HM1404, HM108, HM1343, HM1022, HM2211, HM6711) were
attempted to transfer hull-less seed trait, however success was achieved in 5.57% crosses
only. The fruit setting, number of seeds/fruit, seed germination and survival of F1 seedlings
were high when C. pepo was used as female parent in cross HLP36 × HM1343 (66.67%,
15.00, 45.24% and 49.48%), followed by HLP36 × HM1022 (22.73%, 23.00, 44.35%,
47.06%) and HLP44 × HM1022 (13.79%, 12.00, 45.85%, 45.46%), respectively. However, in
reciprocal crosses (C. moschata × C. pepo) success rate was very poor, which was overcome
through in vitro ovule culture after 17 to 19 days of pollination in cross HM1343 × HLP53
and HM6711 × HLP72. The hybridity of putative Cucurbita interspecific hybrids was
confirmed through molecular, morphological and micromorphological markers and their
results were found to be highly consistent. The phenotyping of F2 populations of interspecific
crosses confirmed the transfer of hull-less seed trait. Findings of the present study will
facilitate in genetic dissection of candidate gene(s) and to enhance productivity, improve
quality and multi-season cultivation of hull-seeded pumpkin in India.
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Citation
Barinder Kaur (2020). Molecular mapping and interspecific hybridization for hull-less seed trait in pumpkin for value addition in food processing industry (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.