EXPLORING MICROBIAL ENDOPHYTES AS POTENTIAL ANTAGONISTS AGAINSTS Colletotrichum gloeosporioides (PENZ.) CAUSING ANTHRACNOSE OF MANGO
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Date
2017-06
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University of Horticultural Sciences, Bagalkot (COLLEGE OF HORTICULTURE, GKVK POST, BENGALURU)
Abstract
Anthracnose caused by Colletotrichum gloeosporioides (Penz.) is the major disease of mango which occurs both as a pre harvest and post-harvest disease. Endophytes are microorganisms which colonize healthy plant parts intercellularly and or intracellularly without causing any outward symptoms of the disease. In this study, endophytic fungi and bacteria were isolated from ten different varieties of mango viz., Alphanso, Totapuri, Neelum, Anfas, Willard, Badam Model, Khaderi, Pancharasi, White Sari and Kisan Bhog. The isolated endophytes were screened for their antagonistic activity against the pathogen. A total of 35 endophytic fungi and 20 endophytic bacteria were isolated from ten different varieties of mango. Morphological characterizations of endophytes were done and among the fungal isolates, and most of them were nonsporulating. Among all the fungal isolates 17 were had septate mycelium and 18 showed aseptate mycelium and among bacterial isolates all of them were gram positive. All the bacterial isolates exhibited negative reaction for indole test. Among all the fungal isolates screened for their antagonistic activity, EFWS-A, isolated from the stem tissues of Willard showed maximum inhibition of 83.70% followed by isolates from Alphonso leaf and stem tissue EFAL-C and EFAS-D with inhibition of 72.32 % and 71.11% respectively. Among all the endophytic bacterial isolates from different mango varieties, EBANL-1 isolated from the leaf tissue of Anfas showed maximum inhibition of 56.67% followed by EBPS-1 (55.48%) isolated from stem tissue of Pancharasi. Hence, endophytic fungi EFWS-A and endophytic bacteria EBANL-1 can be identified and exploited for further use as a biological agent.