A STUDY ON SHORTENING HYBRIDIZATION CYCLE IN ROSE: MECHANISM AND REGULATION

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Date
2018-07
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University of Horticultural Sciences, Bagalkot. (College of Horticulture, Bengaluru).
Abstract
The present study was carried out at ICAR-IIHR, to study the effect of pre- crossing treatment on hybridization, pre-sowing treatment on seed germination and to understand the level of endogenous hormone during different physiological stages of hip development. The experiment on pre-crossing treatment was conducted with nine pre-crossing treatments using different combinations of gibberellic acid (0, 100, 200 ppm) and boric acid (0, 250, 500 ppm). Hybridization during August-December months with the application of either GA3 at 100 ppm or boric acid at 500 ppm prior to crossing enhanced the number of seeds per hip and could minimize the days to seed set. Levels of endogenous hormones was estimated during different physiological stages of hip development to understand the role of hormones in regulating the seed germination process in rose. GA3 content was found to be maximum and ABA was minimum during 28-35 days after pollination. Effect of various seed treatments was evaluated on seed germination in five rose genotypes. Seed germination percentage varied with the genotypes, treatments and incubation temperature. Among the different incubation temperature, maximum seed germination (19.11%) was observed at 150C and minimum (2.66%) at 100C. Among different treatments, maximum seed germination of 41.33 per cent was observed in IIHRR 3-7-12 with the pre-sowing treatment of mechanical seed scarification by stirring in mixer-grinder. Based upon estimation of endogenous hormone concentration, two stages of seed development were identified one at 28-35 and another at 63 days after pollination, when GA3 as well as IAA were observed to be high and ABA was low. Our study suggested the possibilities of improving the seed germination by harvesting the seeds at these selected stages.
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