Evaluation of ameliorative effect of selenium on carbendazim induced endocrine disruption in male goats
Loading...
Files
Date
2011
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
GADVASU, Ludhiana
Abstract
In the present investigation, ameliorative effect of selenium, an antioxidant, on oral sub chronic toxicosis of carbendazim was assessed by studying various indices of antioxidant defense system, testosterone cyclicity, histopathology and immunolocalization of steroidogenic enzymes. Bucks were randomly divided into four groups of four animals each. Group I served as control, Group II was orally administered carbendazim at the dose rate of 50 mg/kg body weight for 90 consecutive days. Group III was orally administered selenium in the form of sodium selenite at the dose rate of 0.05mg/kg body weight for 90 consecutive days. Group IV was orally administered carbendazim along with selenium at same dose rates. Prolonged administration of carbendazim produced oxidative stress as evidenced by increase in lipid peroxidation and decline in total antioxidant capacity. The increase in the activity of anti oxidant enzymes was not sufficient to prevent pesticide induced oxidative stress. The the mean testosterone concentration and testosterone pulse amplitude was significantly less after carbendazim treatment whereas there was no significant change in pulse frequency. Carbendazim toxicosis caused a severe damage to histological architecture of testis, marked reduction in numbers of Leydig cells and there was extremely weak immunoreactivity for 17 α hydroxylases and 3β hydroxysteroid dehydrogenase (3β HSD) - key enzymes in testosterone production. The co-administration of selenium produced ameliorative effect to some extent.Thus from the findings of present investigation it can be concluded that carbendazim can act as endocrine disruptor and supplementation with selenium have some protective effect against the endocrine disrupting potential of carbendazim in bucks