PROFILING OF LOCAL BASMATI GERMPLASM FOR BACTERIAL BLIGHT RESISTANCE AND GRAIN QUALITY TRAITS
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Date
2020-01-24
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Division of Plant Breeding & Genetics Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu Main Campus, Chatha, Jammu
Abstract
The present study entitled “Profiling of local Basmati germplasm for Bacterial Blight Resistance and grain quality traits” was carried out during crop season Kharif 2018 at the experimental area and molecular breeding laboratory of Division of Plant Breeding & Genetics; and molecular laboratory of School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Chatha, Jammu. The objectives were to identify genotypes having good Basmati quality traits and enhanced resistance to bacterial blight. The material for the present study consisted of 93 germplasm lines along with 6 checks collected from Division of Plant Breeding & Genetics and School of Biotechnology. These genotypes were evaluated during Kharif 2018 in which 21 day old seedlings were transplanted in three rows with spacing of 30 x 20 cm. Analysis of variance for various morphological, phenological, yield attributes and quality parameters showed significant variation for all the characters. All the genotypes were screened for bacterial blight under natural field conditions; and under green house conditions using leaf clipping technique (Kauffman et al., 1973). Scoring was done after 14 days of inoculation following Standard Evaluation System for Rice (SES) IRRI, 1998. All the 93 germplasm lines along with 6 standard checks were selected for bacterial blight screening with gene specific primers viz, RG 136 (xa13) and pTA 248 (Xa21). Among these germplasm lines, 58 were found to have Xa21 gene with band size 1300 bp and 57 were found to possess xa13 gene with band size 800 bp. Out of the total 93 genotypes, 46 genotypes possessed both the genes, whereas 30 genotypes lack both the resistance genes. It was observed that most of the genotypes carrying both the genes (xa13 and Xa21) showed disease score of “1” and “3”. However, genotypes carrying one gene (either xa13 or Xa21) showed disease score of “5” and “7”. Therefore, it is observed that lines carrying two genes have the potential to more resistance to bacterial blight pathogen than single gene. These identified lines can serve as potential donor for the transfer of bacterial blight resistance genes into elite basmati varieties and the varieties having both the resistance genes coupled with higher yields can be used as varieties after testing in yield trails.