EXPRESSION OF ANGIOPOIETIN-1 (ANPT-1) AND AROMATASE (CYP19) IN OVARIAN ANTRAL FOLLICLES OF MALABARI AND ATTAPPADY BLACK BREEDS OF GOATS.
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Date
2019
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College of Veterinary and animal Science,Mannuthy
Abstract
The expressions of ANPT-1 and CYP19 genes in small and large follicles
were studied in Malabari and Attappady Black breeds of goat. Animals (n=6) were
selected from each breed and given PGF2α, after confirming oestrous by detecting
visible external heat signs and open cervix using vaginal speculum, and ultra sound
scanning was done in the animals for one entire cycle and observed the ovarian
activities. We noticed a significant difference in the exhibition of heat signs
between two breeds with a greater mean [(± S.E) 12±1.88] in Malabari than
Attappady black breed [with a mean (± S.E.) 5.67 ±1.12]. On detecting the next
heat, scanning was done from day 15 onwards and the animals were slaughtered on
reaching pro-oestrous stage and the ovaries and blood samples were collected.
Studied the expression pattern of ANPT-1 and CYP19 in different categories of
ovarian antral follicles and estimated the serum oestradiol and progesterone
concentration by RIA. Significant difference was found in serum oestradiol
concentration between Malabari [mean (± S.E) 44.23 ± 7.00 pg/mL] and Attappady
black breed [mean (± S.E) 13.95 ± 4.35 ng/mL], with no significant difference
detected in serum progesterone concentration between breeds. The expression of
ANPT-1 and CYP19 in follicles at different developmental stages within and
between breeds failed to show any significant difference. The expression of ANPT1 was found to be decreased in large follicles of both breeds compared to small
follicles whereas the expression of CYP19 was found to be increased in the large
follicles of both breeds compared to small follicles. Hence it is concluded that
changing pattern of ANPT-1 and CYP19 expression have a definite role in
controlling the follicular development in these two breeds.