CHARACTERIZATION OF ENDOPHYTIC FUNGI IMPARTING SALINITY STRESS TOLERANCE IN RICE (Oryza sativa L.)
Loading...
Files
Date
2019-08-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE
Abstract
Endophytes are the microorganisms (fungi or bacteria) that lives inside the plant
tissue symbiotically. They influence on plant growth and impart abiotic and biotic stress
tolerance. In this study, twenty fungal isolates were screened (in-vitro) for salinity stress
tolerance at different concentration of NaCl (0.50, 1.00, 1.50, 2.00 and 2.50M). Among 20
isolates, the four isolates viz., P-9, P-10, P-26 and P-35 showed growth at 2.50 M
concentration. These four isolates were identified by comparing ITS region sequence
amplified by PCR using ITS1 and ITS4 primers. These isolates were identified by using
the sequence data available at National Centre for Biotechnology Information (NCBI,
USA). The four fungal isolates (P-9, P-10, P-26 and P-35) were identified as Fusarium
acuminatum, Fusarium tricinctum, Fusarium sp. and Setosphaeria rostrate respectively.
The identified fungal isolates were evaluated by treating them with pre-germinated paddy
(IR-64) seeds using blotter paper and paper towel methods. In both the methods, the two
endophytes (Fusarium sp. and Fusarium tricinctum) showed increased root and shoot /
seedling length compared to others. Therefore, these two endophytes were selected for pot
experiment under greenhouse conditions. In the pot culture study, the endophytes
inoculated plants showed significantly increased plant height, number of leaves and
number of tillers besides increasing the chlorophyll content as compared to un-inoculated
plants under salinity stress. This study, suggested that the inoculation of endophytes can
impart tolerance to salinity stress and improves plant growth.
Description
Keywords
null