EFFECT OF SUPPLEMENTING NANO FORMS OF COPPER AND COBALT ON RUMEN MICROBIAL BIOMASS PRODUCTION
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Date
2018
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Abstract
A research was conducted with the objective to produce nano particle source of copper and nano particle source of cobalt, characterize them, and study the effect of supplementing them on rumen fermentation. Nano particle source of copper and cobalt were synthesised through physical method using ball mill. The product yield of nano particle source of copper and cobalt were 50 g/hour and 10 g/hour repectively. The particle size and zeta potential of the synthesised nanoparticle source of copper and cobalt were (14.28 nm and – 11.03 mV) and (14.67nm and -12.73 mV) respectively. The particle shape for both nano particle source copper and cobalt were spherical, which was confirmed by TEM (Transmision Electron Microcope). Through FTIR (Fourier Transform Infra Red spectroscopy) and XRD (X Ray Diffraction), it was proved that the synthesised nano particle source of copper and cobalt had no impurities.The percentage of copper and cobalt in the synthesised nano particle source of copper and cobalt were 23.26 % and 19.57 %, respectively.The in vitro cytotoxicity assay of both nanoparticle source of copper and cobalt on VERO cell line revealed that the safe level for inclusion of copper and cobalt nanoparticles in dairy cattle ration was upto
1.81g/kg of concentrate. The effect of nano particle source of copper and cobalt as critical nutrients compared to their mega particle sources on rumen fermentation and rumen microbial protein production were studied in gruel / rice based feeding regimen through In vitro Gas Production Technique. Three in vitro studies were carried out. In first in vitro study, the inclusion level of mega copper and nano particle source of copper to be used for combination study were determined as 15 μg each respectively. In second in vitro study, the inclusion level of mega cobalt and nano particle source of cobalt to be used for combination study were determined as 0.5μg and 1.5 μg respectively. In third in vitro study, combination of nano/mega copper and nano/mega cobalt supplementation was studied for effect on in vitro rumen fermentation. It was concluded that combination of nano copper and nano cobalt revealed significantly (p<0.01) higher microbial biomass production, higher protozoal and bacterial count, higher total VFA and lower percentage of methane production on comparing with other treatments. Conclusion of overall study was supplementing nano forms of copper and cobalt showed higher rumen microbial biomass production.
Description
TNV_TH_MVM 16011_2018
Keywords
Veterinary Science