ISOLATION AND CHARACTERIZATION OF ANTIMICROBIAL RESISTANT CAMPYLOBACTER SPP. FROM CHICKEN MEAT SOLD IN DIFFERENT ZONES OF CHENNAI
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Date
2018
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Abstract
A study comprising of three parts was conducted, firstly, to assess the contamination of thermophilic Campylobacter spp. in retail chicken meat sold in Chennai Corporation limits, secondly, to determine the antimicrobial resistance pattern of the obtained Campylobacter spp. isolates by using the phenotypic method of Kirby- Bauer disc diffusion and genotypic characterization for antimicrobial resistance genes using Polymerase Chain Reaction. The third part consisted of assessing the biofilm forming ability by Congo red agar assay and micro titer plate assay. For the first part of the study, samples collected randomly from all fifteen zones of Chennai Corporation were screened for the presence of Campylobacter spp. by conventional culture method and further confirmed by Polymerase Chain Reaction. After confirmation of the Campylobacter genus, the isolates were identified to the species level by using species specific primers and also by multiplex PCR. The results of prevalence study showed that 66.67 percent (218/327) of the chicken meat portions
examined were showing presumptive colonies for Campylobacter spp. Molecular identification revealed that 25.38 percent (83/327) of the total samples were positive. Breast meat portions showed high levels of contamination in both assays.In the second experiment, a representative portion of isolates consisting of Campylobacter jejuni, Campylobacter coli and mixed isolates containing Campylobacter jejuni and Campylobacter coli were assessed for resistance pattern against ciprofloxacin, tetracycline, cefoxitin, tylosin and colistin among which ciprofloxacin, tetracycline and cefoxitin resistance was observed. The presence of various antimicrobial resistance genes corresponding to fluroquinolones, betalactams, tetracyclines and erythromycin resistance were identified. The third part of the study revealed that the isolates obtained from this region were potent biofilm formers showing black discoloration on Congo red agar. Hence the complete study demonstrates the presence of antimicrobial resistant and biofilm forming ability of Campylobacter spp. which poses a major zoonotic threat to the public health in contaminated chicken meat portions.
Description
TNV_TH_MVM16058_2018
Keywords
Veterinary Science