Effect of Two Different Culture Media on Developmental Rate of Bovine Embryos invitro
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Date
2020-06
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Abstract
In the present study, effect of two different culture media on the developmental
competence of bovine oocytes was studied. For the study a total of 1110 oocytes were
collected using ovum pick-up (OPU) technique and the average oocyte yield was 8.34 ±
0.23 per OPU session per animal. The oocytes were graded as A, B, C and D grades based
on the layers of compact cumulus cells surrounding the zona pellucida. Among this only
A, B and C grade oocytes were used for in vitro maturation. The oocytes were matured in
TCM 199 media supplemented with 10 per cent FBS, 1 g/ml Folltropin (Bioniche,
Canada), 0.02 IU/ml LH, 1 g/ml estradiol with addition of 100 μM cysteamine, 10 ng/ml
EGF, 100 ng/ml IGF, 10 μg/ml insulin, 5.5 μg/ml transferrin and 5 μg/ml selenium and the
overall maturation rate was 91.30± 1.27 per cent. The presumptive zygotes were cultured
in two different culture media viz. i) two step sequential synthetic oviduct fluid (SOF)
media and ii). single step potassium simplex optimised medium (KSOM). The mean (±
SE) cleavage, 4 cell, 8 cell and morula development rate was 73.04 ± 1.50 and 79.01 ±
1.34 per cent, 54.88 ± 1.76 and 60.69 ± 1.89 per cent, 35.89 ± 1.57 and 44.67 ± 1.62 per
cent and 19.18 ± 1.10 and 25.36 ± 1.37 per cent in SOF media and KSOM, respectively.
Based on the cleavage and embryo development observed, it was concluded that KSOM
could be better than SOF media for in vitro production of bovine embryos.
Description
TNV_IJCMAS_2020_9(6)1563-1572
Keywords
Veterinary Science