A novel doorstep canine haemoprotozoan diagnostic service – A preliminary survey in Chennai
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Date
2020-02
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Abstract
Canine vector-borne diseases pose a major health problem in dogs globally. A door-step diagnostic
service for diagnosis of canine haemoprotozoan diseases by polymerase chain reaction (PCR) was launched
in collaboration with the Translational Research Platform for Veterinary Biologicals (TRPVB). The aim of
this service was to provide door-step diagnosis to practicing veterinarians by collecting the samples from their
clinics and providing results with 24 hours. Initially 2 PCRs were done to identify the presence of Babesia
and E. canis genomes through amplification of 619 bp and 377 bp products respectively. Then a nested PCR
was done independently on all samples to identify the Babesia species, Babesia gibsoni and / or Babesia canis
genomes through amplification of 195 bp and 197 bp nested PCR products respectively. Of the 55 samplestested, 17 samples were positive for Babesia genus specific primers and 4 for E. canis. When nested PCR was
used for detection of Babesia sp., 31 was positive for Babesia gibsoni and 10 for Babesia canis. Six samples
were positive for both Babesia gibsoni and Babesia canis. Three samples were positive for both Babesia
gibsoni and E. canis. None of the samples were positive for Babesia canis and E. canis. While 35 samples
were positive for either Babesia in nested PCR only 17 were positive in single PCR. This suggests that use
of nested PCR is more sensitive and hence more applicable in clinical settings in both Babesia identification
and speciation.
Description
TNV_20thMVC_PP_Feb-2020_PA60
Keywords
Veterinary Science