Genotype and gene frequencies of A1 and A2 β-casein genes in native and cross bred cattle of Tamil Nadu
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Date
2020-02
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Abstract
Beta-casein (CSN2) constitutes up to 45% of the casein of bovine milk. Among the 12 known genetic
variants of β-casein gene, the most common forms of β-casein in dairy cattle breeds are A1 and A2 β-casein
variants. The present study aims to study the β-casein genotypes of the milk samples (506) that were received
at TRPVB facility from various organized farms of Tamil Nadu. This study included native cattle breed
(139) and cross bred (367) cattle. The DNA was extracted from samples received on whatman cards and
the genotype was determined using allele specific PCR. The results of the study showed that the genotypic
frequency for A1/A1, A2/A2 and A1/A2 of native cattle breed was 0%, 78% and 22%, respectively. While the
genotypic frequency for A1/A1, A2/A2 and A1/A2 of cross bred cattle was 4%, 63% and 33%, respectively.
The gene frequency of β-casein in crossbred cattle was 20% (A1) and 80% (A2); whereas the native cattle
breed exhibited A1 allele frequency of 11% and A2 allele frequency of 89%.
Description
TNV_20thMVC_PP_Feb-2020_PA07
Keywords
Veterinary Science