Detection and molecular characterization of avian infectious laryngotracheitis virus isolated from a recent outbreak in a breeder flock
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Date
2020-02
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Abstract
Avian Infectious laryngotracheitis (ILT) is an economically important poultry diseasecaused by
gallidherpesvirus I (GaHV-1), a double stranded DNA virus of 155 Kb in size belongs to the genus Iltovirus,
family herpesviridae.A breeder flock situated near Chittoor, consisting of 12,945 birds was reported with
the problem of severe respiratory distress and mortality. The Clinical signs observed were pump handle
respiration and nasal discharge. Morbidity and mortality rate observed were 70% and 12% respectively. On
post mortem examination haemorrhage and caseous plug were noticed in the trachea. DNA extracted from
suspected tracheal samples were screened by PCR based on ICP4 gene in which amplicon specific to ILTV of
635bp were obtained. ILTV positive samples were isolated by chicken embryo inoculation via chorioallantoic
membrane (CAM) route of inoculation. Characteristic pock lesion was observed after third passage. DNA
extracted from the CAM was amplified with glycoprotein J gene primers. Amplicon size of 1.39 kb was
amplified and sequenced. Sequence analysis revealed that the current isolates belong to haplotype II and
phylogenetically related to CEO high passage vaccine strain, USA virulent , Italy virulent , China virulent,
Australian virulent and Russian virulent ILT strain.
Description
TNV_20thMVC_PP_Feb-2020_OP58
Keywords
Veterinary Science