A pilot study for diagnosis of bovine subclinical mastitis at the field level by TRPVB “masti-detect” meter
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Date
2020-02
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Abstract
Existing diagnostic methods for subclinical mastitis have limited application at farm-level. The current
study aimed at testing and validating a rapid, user friendly and cost-effective mastitis diagnostic “Masti-
Detect” meter developed at TRPVB, TANUVAS for diagnosis of subclinical mastitis. “Masti-Detect” detects
changes in electrical conductivity of milk. Milk samples from a total of 120 cattle and 30 buffalo from
livestock farm complex, VC & RI, Orathanadu and 60 cattle and 10 buffalo from clinically suspected mastitis
cases which were brought to Veterinary Clinical Complex, VC & RI, Orathanadu were screened for mastitis
using “Masti-Detect”, bacterial isolation and PCR. Healthy cattle and buffaloes were included as negative
control. For “Masti-Detect” 5 ml of bovine milk was collected in the cup provided and probe of the meter was
inserted and the readings were recorded immediately. A value of ≥ 6.0 was considered positive for subclinical
mastitis. By “Masti-Detect”, in farm animals, 20.8% cattle and 33.3% buffalo were positive whereas in
clinically suspected cases, 50% cattle and 80% buffalo were positive for subclinical mastitis. By isolation andidentification technique, in a farm 16.7% cattle and 33.3% buffaloes were positive and in clinical cases, 35.7%
cattle and 50% buffalo were positive. All the isolated organisms were confirmed as S.aureus by PCR. Healthy
animals were negative for mastitis by “Masti-Detect” and by isolation. It is concluded that “Masti-Detect”
can be effectively used for early diagnosis of subclinical mastitis at field level.
Description
TNV_20thMVC_PP_Feb-2020_OP10
Keywords
Veterinary Science