ASSOCIATION OF IGF1 GENE POLYMORPHISM WITH GROWTH TRAITS IN SHEEP BREEDS
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Date
2019-12
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Abstract
A total of 297 sheep were genotyped for polymorphism in the IGF-I gene in Mecheri and Madras
Red sheep breeds. In the PCR reaction the primers used were 5’ ATTACAGCTGCCTGCCCCTT 3’ and 5’
CACATCTGCTTACACCTTACCCG 3’. A 265 bp long IGF1 (exon1) gene PCR product was genotyped for
polymorphic pattern using SSCP method. The PCR-SSCP analysis of exon1 IGF-I revealed three distinct
patterns viz., AA, AG and GG. The frequencies were 269 for pattern AA, 20 for pattern AG and 8 for pattern
GG. The effects of genotype on the birth weight and weight gain at one month, two months, three months,
six months, nine months and twelve months of age for IGF1 - SSCP genotypes were analyzed. A significant
effect (P<0.05) of genotypes on weight gain was recorded only at twelve months of age in this study. The
twelve months weight gain of AA genotype (16.43 ± 0.56 kg) was significantly higher than that of AG and GG
genotypes (14.81 ± 1.14 kg and 13.80 ± 1.82 kg).
Description
TNV_35thVCRI_CA_Dec-2019_SRSO-20
Keywords
Veterinary Science