Fluorescence Activated Cell Sorting (FACS) of CK15 Cells in Canine Hair Follicular Tissue
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Date
2020-02
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Abstract
Hair follicle is an ideal system for studying the biology and regulation of
adult stem cells. Multipotent stemness of the canine hair follicle stem cells
(cHFSCs) were detected by immunostaining the cells with positive HFSC
surface markers like Anti-cytokeratin-15 (CK15) and CD200. In this study,
canine hair follicular cells were cultured in Williams’E media
supplemented with growth factors. The primary cultures of the hair follicles
were subjected to Fluorescence Activated Cell Sorting (FACS) and then
cells were immunostained with CK15 and DAPI. In primary culture,
18.28% of cells were positive for CK15 and sorted out by FACS. The
positive expression of CK15 transcription factor was observed with DAPI
as a counter stain in the in-vitro cultured cHFSCs. These results indicated
that the cells in the canine hair follicular bulge region possess the stemness
and act as a precursor and involved in hair cycle.
Description
TNV_IJCMAS_2020_9(2)2929-2934
Keywords
Veterinary Science