Antiproliferative effect of Tinospora cordifolia on MDA-MB-231cell line
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Date
2012
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Abstract
The present study was taken up to explore the antiproliferative activity of aqueous and hydro-alcoholic extracts of Tinospora cordifolia in MDA-MB-231human breast cancer cells each at three different doses viz. 200, 400 and 600 μg/ml. Their influence on the proliferation of cells were analysed by estimation of cell count, cell viability and cytotoxicity using trypan blue dye exclusion method and MTT assay, detection of apoptosis by flow cytometry and expression of apoptosis related genes. The expression of telomerase was determined by using RT-PCR. Both the extracts produced significant inhibition of cell count and cell viability, with maximum effect being noticed at the dose level of 600 μg/ml. Induction of apoptosis was shown by changes in nuclear morphology and formation of apoptotic bodies, though there was no DNA laddering in agarose gel electrophoresis. There was an expression of the apoptosis related genes in the treated groups. Flow cytometric analysis revealed an increase in the percentage of apoptotic cells dose-dependently, after treatment with both the extracts of the plant. Telomerase RNA and hTERT mRNA was expressed in the control but not in the treated groups by RT-PCR analysis. These results suggest that aqueous and hydro-alcoholic extracts of Tinospora cordifolia could suppress cell proliferation and induce apoptosis in MDA-MB-231cell line and act as effective natural anticancer agents in mammary tumour.
Description
TNV_IJCS_2012(2)63-71
Keywords
Veterinary Science