Microsatellite- based phylogeny of Indian sheep breeds
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Agricultural Research Communication Center
Abstract
Genetic diversity of south Indian meat type breeds was investigated by means of 15 ovine microsatellite markers.
All used microsatellites amplified well and exhibited polymorphisms. A wide range of genetic variability was
observed as allele varied from 4 to 10 in Madras Red; 4 to 10 in Mecheri; 4 to 12 in Pattanam; and 3 to 12 in Nellore
sheep breeds. The mean number of alleles observed in Madras Red, Mecheri, Pattanam and Nellore breeds were
6.5, 6.7, 7.0 and 7.2 respectively. Average observed and expected heterozygosities over the different breeds were
0.23 to 0.87 and 0.347 to 0.827 respectively. The most diverse sheep breed was Nellore breed and the least diverse
breed was Madras Red, which had the lowest total number of alleles (na=98) and lowest average observed (Ho=0.576)
and expected (Ho=0.594) heterozygosities. The multi-locus mean between-population variability (FST) values
indicated that about 5.60% of the total genetic variation was explained by population differences, whereas, the
remaining 94.40% is due to differences among individuals within breeds. The values of FIT in the sub population
for the most of markers were positive which showed the deficiency of heterozygotes. The DA genetic distance and
FST distances between pairs of breeds revealed that the lowest distance was between Madras Red and Mecheri and
the highest between Mecheri and Nellore breeds. Analysis of individual genotypes provided valuable information
for understanding intra and inter-population genetic differences and helps in planning genetic improvement and
conservation strategies for further improvement and sustainable utilization.
Description
TNV_IJAS_2015_85(11)1209-1214
Keywords
Veterinary Science