Study on Bio chemical changes and management strategies against Bacterial Leaf Blight Disease.
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Date
2019
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Department of Mycology and plant pathology Institute of Agricultural Sciences Banaras Hindu University, Varanasi
Abstract
Rice (Oryza sativa L.) belongs to family poaceae, is one of the most important cereal crops of the world and especially of Asian countries. Rice crop is attacked by many pathogens. Bacterial leaf blight is one of the major diseases of rice that is caused by Xanthomonasoryzaepv.oryzae (Xoo) bacteria.Pathogenicity test of different five isolate (viz., BLB-1, BLB-2, BLB-3, BLB-5 and BLB-10 ) of BLB has been carried out on susceptible HUR 4-3 local variety under glass house condition.All the isolates gave positive test of Catalase and Methyl red test while there was a variation in Gelatin liquefaction, Phosphate solubilization and Potassium hydroxide test. All isolate gave negative result when tested for ammonia production.Eleven botanicals have tested in various solvent viz., aqueous, acetone and methanol extract. Most effective botanical was onion bulb extract organic solvent, acetone extract of neem leaf was most effective.Among antagonistic effect of different bioagents against Xoo, Aspergillus spp. was found most effective and minimum inhibition zone produced by Harposporiumspp..In-vitroefficacy of antibiotics also observed and found that streptomycin sulfate at 1000 ppm was best among all the treatments while Kanamycin sulphateat 750 ppm found most effective followed by Ampicillin sodium at 1000 ppm. Screening of 571 lines/genotype of rice (NSN-1, DSN and NHSN) were provided by IIRR, Hyderabad in AICRIP programme and evaluated on SES scale. Out of 354 NSN-1 entries, 6 lines was highly susceptible to BLB disease and 85 lines gave susceptible reaction and 220 lines are moderately resistant to BLB disease. In Donor Screening Nurseries 28 lines showed susceptible reaction, while 56 lines are moderately resistant to BLB disease and 6 lines were found resistant to BLB. Among 127 entries screened for NHSN 20 lines showed susceptible reaction, while 97 lines as moderately resistant and only 10 lines were found resistant to BLB pathogen, with 3 score on SES scale. After evaluation of popular eight rice varieties, of Eastern Uttar Pradesh HUR 3022 and Improved Samba was found resistant gave score of 3 on SES score. SPAD reading for chlorophyll content showed Improved samba variety has highest chlorophyll content and least affected by inoculated BLB pathogen followed by HUR 3022 variety while MTU 7029 showed reduction in chlorophyll content as measured by SPAD meter, 45.12% reduction in SPAD reading between un-inoculated and inoculated variety of MTU 7029.
assessment of enzymatic activity of PAL, PO, PPO and phenol content exhibit maximum in the Improved samba variety minimum in the rice variety Swarna (MTU-7029).
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Bio chemical