STUDY OF PADDY STRAW BIODEGRADATION BY SUCCESSIVE INOCUATION OF TRICHODERMA AND YEAST (SACCHAROMYCES)
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Date
2019
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Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.)
Abstract
In present investigation paddy straw pre-treated with 1% NaOH solution and further neutralized with 10% Hcl up to pH 7. The effect of pre-treatment was analyzed by FTIR analysis. FTIR revealed the degradation of cellulose and lignin of paddy straw further reducing sugar was also estimated by dinitrosalicylic acid (DNS) method in both treated and untreated paddy straw broth. The pre-treated paddy straw was further degraded by inoculation of Trichoderma reesei with 5 treatments (T1-alkali pre- treated rice straw + T. reesei, T2-without pre-treated rice straw + T. reesei, T3- without pre-treated rice straw, T4- without pre-treated sterilized rice straw + T. reesei, T5-alkali pre-treated sterilized straw + T. reesei). Results revealed that best treatment was T5 (i.e. alkali (NaOH) pre-treated sterilized (S) rice straw+ Trichoderma) followed by T2 (Without treatment rice straw (WT)+ Trichoderma) and T4 (Without treatment sterilized (S) rice straw + Trichoderma). To detect bioethanol production Saccharomyces cerevisiae was inoculated into treatment T4 and T5 after killing fungal spore by exposing at 50 oC. FTIR analysis revealed specific peak of cellulose around 3400 to 3200 and 2900 cm-1 and have been assigned as the O-H stretching of hydrogen bond and C-H bonds, respectively. Results shows that these peaks were reduced in pre-treated as compare to untreated paddy straw and confirm the degradation of cellulose, similarly peaks around 1600 cm-1 is assigned lignin and this peak also reduced in treated straw. The ethanol production was confirmed by GC-MS in treated and untreated straw after yeast inoculation.
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