Detection of Mycoplasma galliseptium infection in chickens from Tamil Nadu State of India
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Date
2019-01
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Abstract
Mycoplasma gallisepticum (MG) is one of the major respiratory tract pathogens affecting chickens. It causes Chronic
Respiratory Disease (CRD) among chickens of various age groups. The present study describes the isolation, molecular
detection and histopathological changes associated with CRD in chickens. A total of 790 samples viz., trachea, lungs and
air sacs were collected from chickens showing the symptoms of CRD from different parts of Tamil Nadu state. All the
samples were processed for isolation and molecular detection of MG. A total of 91 samples were found positive by isolation
and 105 samples were found positive through MG specific PCR targeting 16S rRNA gene. The histopathological changes
in tissue samples of trachea, sinuses, air sacs and lungs collected from naturally infected M. gallisepticum infection were
suggestive of subacute to chronic nature of infection. Though isolation is considered to be a gold standard, still PCR is a
rapid, sensitive and cheap method for early diagnosis of MG which can help poultry farmers to avoid severe economic loss.
Description
TNV_IJAR_2019_53(1)1-4
Keywords
Veterinary Science