EXTRACTION AND AMPLIFICATION OF LEPTOSPIRAL DNA FROM KIDNEY TISSUES OF RAT
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Date
2007
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Veterinary Association
Abstract
Most human outbreaks of leptospirosis
are confined to developing tropical and
subtropical countries (Faine et al. 1999).
Reservoirs of infection include chronically
infected wild and domesticated carrier animals.
Transmission is effected directly from
contaminated urine or indirectly by entry from
environments that permit leptospiral survival.
Rodents contributes for 60% of transmission
of the disease (Ratnam et aI., 1987).
Maintenance of leptospires in chronically
infected carrier animals makes the control of
the disease difficult and results in significant
economic losses in cattle, pigs and horses
(Donahue, 2001). isolation of leptospira is a
long term process. For early diagnosis, in the
present study the usefulness of PCR in
amplification of leptospiral DNA from kidney
tissues of rat employing two methods of DNA
extraction is reported.
Description
TNV_IVJ_2007_84_908-910
Keywords
Veterinary Science