Demonstration of Osseous Tissue in Quail Embryo by Alizarin Red Technique
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Date
2018-06
Journal Title
Journal ISSN
Volume Title
Publisher
Excellent Publishers
Abstract
The embryonated Namakkal quail eggs were collected from the hatchery at tenth
and fourteenth day of incubation. The eggs were opened by standard technique
and the embryos were collected and stained by alizarin red staining technique to
demonstrate the osseous tissue development. The scapula, coracoid, clavicle and
proximal bones of wing were well developed in both tenth and fourteenth day
embryos. But the fusion of pelvic girdle with the synsacrum and the distal bones
of wing were completely developed only in fourteenth day embryo. The femur,
tibia, fibula, tibiotarsus, tarsometatarsus and digits were found like a rod of bones
without distinct joints in tenth day embryo. Skull bones, vertebral bones and ribs
were fully ossified in fourteenth day quail embryos. But the sternal ossification
was not observed by alizarin red staining technique in both the quail embryos. A
ring of scleral ossicles in the sclera around the margin of cornea were distinctly
observed in both the embryos studied.
Description
TNV_Ijcmas_2018_7(6)2286-2289
Keywords
Veterinary Science