Evaluation of Sperm Viability and Acrosomal Integrity by Flow Cytometry Analysis in Jersey Crossbred Bulls
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Date
2017-10
Journal Title
Journal ISSN
Volume Title
Publisher
Excellent Publishers
Abstract
Flow cytometry based evaluation of sperm parameters is advantageous over the traditional
microscope based evaluation. Many sperm parameters can be measured within short time
period and with high accuracy. For this study, semen from six Jersey crossbred bull were
collected. Semen parameters like sperm for viability and acrosomal integrity were
analyzed by flow cytometry. Sperm for viability measured by the propidium iodide
staining indicated that Bull No: 3 have the highest number of viable spermatozoa with
82.89 per cent followed by 82.18, 80.26, 79.43, 75.20 and 70.73 for Bull No: 1, 5, 2, 6 and
4 respectively. Acrosomal integrity of the sperm sample were determined by fluorescein
isothiocynate (FITC) labelled with Peanut agglutinin (PNA) staining. The percentage of
viable sperm, non-viable sperm, viable intact sperm, viable damage sperm and viable
damage and non-viable sperm was ranged from 75.12 to 82.28, 17.72 to 30.66, 58.55 to
78.48, 3.80 to 10.80 and 21.52 to 41.45 respectively. Chi’s square analysis of the semen
samples indicated that there was a significant difference among bulls with respect to both
the sperm viability and acrosomal integrity (P<0.01).
Description
TNV_Ijcmas_2017_6(10)87-93
Keywords
Veterinary Science