Identification and validation of molecular markers for MYMV resistance in mungbean (Vigna radiata L.Wilczek)
Loading...
Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Orissa University of Agriculture and Technology, Bhubaneswar
Abstract
Mungbean (Vigna radiata L. Wilczek) is the third most important pulse crop in India. It is a self-pollinated plant with a genome size of 515Mb and it belongs to the family Facbaceae. The crop has already been transformed from a marginal crop to a major crop for its additional benefits like enhancing soil fertility, improving rural household income, expanding employment opportunities, diversifying diets and increasing nutritional security. Mungbean yellow mosaic virus (Begomovirus) is a major exotic disease of mungbean causing leaf discoloration and yield losses. Yellow mosaic occurs across the Indian subcontinent and is a major constraint to cultivation of most warm season legumes, particularly mungbean and urdbean. An annual loss of over US$ 300 million in these crops is incurred as a result of MYMV infection. There is urgent need to develop a resistant variety for YMV. The present investigation was to screen the mungbean land races collected from different geographical regions of Odisha and validate the genotype resistance to MYMV. The experiment was conducted at KVK Sakhigopal, Puri as well as OUAT farm (EB1) during 2016-17 using an augmented design. Phenotypic and genotypic study was conducted in 28 genotypes of mungbean including 04 check varieties. In the field condition, based on the rating scale, individuals were categorized as resistant, moderately resistant, susceptible and highly susceptible individuals and were graded according to the 1-9 arbitrary scale. Mungbean Yellow Mosaic Virus infected plants put forth reduced leaves, stunted peduncle, reduced and curled pods with immature and undersized seeds. Symptoms were more prominent in the genotypes ‘Bargarh-3’, ‘Jharsuguda-1 and ‘Saline 7. A noteworthy difference in yield and yield components were observed among the genotypes tested due to MYMV infection. Out of 10 molecular markers including two SCAR markers tested, six markers (YMV-1, MYMV-583, VR-135, VR-044, VR-078 and DMB SSR-125) were found polymorphic between the resistant and susceptible green gram genotypes studied. In the present study, the validation of the SCAR marker MYMV-583 linked with MYMV resistance gene in different genotypes with known YMD reaction showed consistent association of the marker in all the MYMV-resistant genotypes and absent in the MYMV-susceptible genotypes. Similarly, VR-135, VR-044, VR-078 reported to be linked to MYMIV resistance amplified the respective marker fragment of 200bp in all green gram genotypes and were polymorphic. The markers DMB-SSR-158, DMB-SSR-130, VR-095 and DMB-SSR-080 failed to to differentiate the resistant and susceptible green gram genotypes. Hence, the results confirmed that genotype ‘Makarjhola local’ which was moderately resistant to MYMV can be used in breeding programs. In the present study, markers linked to the resistance gene have more prominently validated using diverse mungbean genotypes. These linked markers will enhance the efficiency and precision of MYMV resistance breeding in mungbean.
Description
Th-5231
Keywords
null