Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation
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Date
2012-08
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Abstract
The objective of this study was to evaluate the effect of supplementation of recombinant leukaemia
inhibitory factor (LIF) in culture media on blastocyst development, total cell number and blastocyst
hatching rates and the reverse transcription-polymerase chain reaction analysis of preimplantation
buffalo embryos to determine whether they contain the LIF-encoding mRNA and its beta receptor
(LIFR ) genes in different stages of preimplantation buffalo embryos. Cumulus–oocyte complexes
retrieved from slaughterhouse buffalo ovaries were matured in vitro and fertilized using frozen buffalo
semen. After 18 h of co-incubation with sperm, the presumptive zygotes were cultured in modified
synthetic oviductal fluid without (control) orwith rhLIF (100 ng/ml). Therewas no significant difference
in the overall cleavage rate up to morula stage however the development of blastocysts, hatching rate
and total cell numbers were significantly higher in the LIF-treated group than control.
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Veterinary Science, Veterinary Physiology