OSMOTIC STRESS INDUCED CHANGES IN LEVELS OF ANTIOXIDANT AND ANTIOXIDATIVE ENZYMES IN CHICKPEA CULTIVARS
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Date
2013
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MPKV, UNIVERSITY LIBRARY RAHURI
Abstract
The present research work entitled, “Osmotic stress
induced changes in levels of antioxidant and antioxidative enzyme
in chickpea cultivars” was carried out with a view to evaluate the
effect of PEG-6000 induced osmotic stress on nine chickpea
cultivars. The four tolerant cultivars viz., ICC-4958, Vijay, Digvijay,
PG-07-52 and five drought susceptible cultivar viz., PG-09103,
ICC-11117, ICCGP-11112, JG-216 and PG-1808 were subjected to
PEG-6000 mild (-0.4MPa) and severe(-1.2MPa) induced osmotic
stress and level of antioxidative enzymes (superoxide dismutase,
catalase, ascorbate peroxidase and glutathione reductase),
antioxidant (ascorbic acid), chlorophyll content and lipid
peroxidation rate were estimated. The level of antioxidative enzyme (superoxide
dismutase, catalase, glutathione reductase and ascorbate
peroxidase) increased in both drought tolerant and susceptible
cultivars of chickpea under both mild and sever osmotic stress
condition as compared to control.
The significantly higher increase in antioxidative
enzyme level under sever stress (-1.2 MPa) as compared to mild (-
0.4 MPa) and control was recorded. The activities of antioxidative
enzymes were higher in drought tolerant cultivars as compared to
drought susceptible cultivars under stress condition and the
highest increase in activities of antioxidative enzymes was recorded
in ICC-4958the best known source of high root biomass, both
under mild and sever osmotic stress.
The significantly increased lipid peroxidation rate under
mild and sever osmotic stress as compared to control was observed
in both tolerant and susceptible chickpea cultivars. The lipid
peroxidation rate was higher in leaves of susceptible chickpea
cultivars than tolerant chickpea cultivars under control, mild and
sever osmotic stress condition.
The significant reduction in ascorbic acid content was
observed under mild and sever osmotic stress as compared to
control in both tolerant and susceptible chickpea cultivars. The
decrease in ascorbic acid content under sever osmotic stress was
more pronounced than mild osmotic stress condition in leaves of
tolerant and susceptible chickpea cultivar. The lower decrease in
ascorbic acid content was recorded in ICC -4958 under both mild
and sever osmotic stress condition. Total chlorophyll content under mild and sever osmotic
stress reduced significantly as compared to control in both
tolerant and susceptible chickpea cultivars. Total chlorophyll
content decreased under sever osmotic stress compared to
control and mild osmotic stress condition in leaves of tolerant
and susceptible chickpea cultivars and was highest in leaves of
susceptible chickpea cultivars. The lower decrease in chlorophyll
content was recorded in ICC-4958 under both mild and sever
osmotic stress condition.
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