CHARACTERIZATION OF POMEGRANATE (Punica granatum L.) CULTIVARS BY MORPHOLOGICAL AND ELECTROPHORETIC
Loading...
Files
Date
1998-07-31
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI-413 722, DIST. AHMEDNAGAR MAHARASHTRA STATE (India)
Abstract
The objectives of the present studies were to characterize pomegranate cultivars by studying theirmorphological features and banding patterns of soluble proteins through SDS-PAGE. The deciduous nature of foliage, erect growing habit, pastel red colour of ovary and flat round fruits were the diagnostic characters to isolate the Cv. Achick Dana. Spinous nature was an important and constant character to identify the various cultivars. The Cvs. Ganesh and G-137 were profusely spiny than rest of the cultivars. The Cv. G-137 can be distinguished from Cvs. Alandi and Ganesh bythe colour of inner surface of calyx, corolla, filament and ovary. The Cv. Ganesh could be identified from other cultivarsby the typical bulbous based hermaphrodite flowers. The Cv. Alandi can be differentiated from rest of the cultivars by the presence of nipple at pedicel end of the fruits. The presence of distinct claw in the corolla isolates Cvs. Alandi, P-26 and Ganesh from other cultivars. Yellow with pinkish tinge colourof style and ovary was unique to Cv. P-26. The deep green colour of new flush, small leaves, the colour of different floral parts and yellow coloured fruits were the distinguishing characters of Cv. Kabul Yellow.The Cv. Mridula can be characterized by the colour of petiole, the characteristic red colour of floral parts and dark redcoloured fruits.Suckering habit, internodal length, arrangement of leaf, adaxial and abaxial leaf colour, leaf shape, number of stamens, the number and size of sepal and petal showed onlyslight variation or no variation between the cultivars and hence these characters were not useful for characterization ofthe cultivars. The bark and rind of pomegranate contain poorquantity of proteins. Qualitative and quantitative variationswere observed in the banding patterns of soluble seed proteinsof the cultivars under study during eletrophoresis. Thepresence or absence of some unique bands distinguish cultivarsfrom each other viz., Cv. Ganesh by the presence of bandnumber 35, Cv. G-137 by band number 31, Cv. Kandhari by 28, Cv. Kabul Yellow by 26 and 29, Cv. Achick Dana by 22 and 23and Cv. Mridula by the presence of bands 4, 7 and 17 and by the absence of bands 1, 3, 6, 10, 16 and 21. As the proteinbanding pattern was not so discriminative enough todistinguish all the cultivars studied, there is further needof going for enzyme eletrophoresis.
Description
Keywords
null