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Kerala Agricultural University, Thrissur
The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively.
Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively.
On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs.
In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc.
During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU).
Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.
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ThesisItem Open Access Participatory action research for renumerative rice production(Department of Agricultural extension, College of Horticulture, Vellanikkara, 2004) Parvathy, S; KAU; Ahamed, PAs with the Complex, Diverse and Risk- prone (CDR) rice systems of many Asian countries, the Kerala state of peninsular India suffers from the fast depleting paddies and the ' high cost- low remuneration syndrome'. Crucial rice technologies have been blamed by stakeholders for the insensitivity to micro farming situations A multidisciplinary stakeholder Participatory Action Research (PAR) of emancipatory type and collaborative mode was done for three years (2001-2004) on identification and prioritization of constraints to profitable rice production to explore the available cost-reducing and productivity increasing technological options. The project focussed on screening of technological modules through farmer participatory field assessment and arrived at locally adaptable and remunerative technology packages. The project also developed and standardised an extrapolatable stakeholder participatory assessment model and protocol. The programme had a blend of extension approaches, research designs and tools like "ex-post facto", benchmark appraisal through Participatory Learning and Action (PLA), exploratory, diagnostic, evaluative, field experiments and analytical studies. The PAR was done in two rice ecosystems (irrigated and rainfe:l) of the midland, laterite belt of Kerala state, India. Fourteen technology modules were fitted into the PAR, under .. , each of the three treatments, viz., farmers' practice, recommended packages of the formal research system and location specific I technology components" jointly decided by the research team extensionists and farmers. Each technology component was subjected to five types of analysis viz., agronomic, statistical, economic, farmers perceptions and reactions, post-trial follow up analysis of adoption in the succeeding cropping seasons. The participatory interventions significantly influenced the level of technical knowledge and extent of adoption of adaptable technology modules, typifying the cognitive impact of an emancipating action research. The short duration red rice varieties "Kanchana" (Ptb 50) and "Kairali" (Ptb 49) proved to be the best first crop and second crop varieties respectively, to replace the ruling cultivars. The technology modules recommended by the formal research system viz., seed treatment with fungicides for the first crop season and Pseudomonas fluorescence for the second crop season were adaptable technologies. The PAR came out with an efficient planting density and crop geometry package (line transplanting; 15 x 10 cm; 67 . , hills/m'; 2-3 seedlings/hill) to replace the conventional planting system. The existing formal recommendations including IPM and INM practices could enhance and combat weeds, pest and diseases thereby enhancing crop yield. Harvesting with self- propelled reaper and threshing with mechanised thresher were cost effective, drudgery alleviating and time saving. The net result of the action research was a set of adaptable technological package for remunerative rice production in the CDR rice production systems. Cognitive and behavioural impact on the participants; and the standard methodology and protocol for participatory technology validation for rice in particular, and for any farm enterprise in general, with extrapolative effect.ThesisItem Open Access Bioactivity of carotenoids from shrimp shell waste(Department of Processing Technology,College of Fisheries,Panangad, 2010) Sindhu, S; KAU; Sherief, P MShrimp processing waste is the single largest industrial waste in the country causing diverse environmental problems. A study was carried out to assess the extractability of astaxanthin from shrimp waste in different organic solvents and vegetable oils. Extraction was tried using wet and dried waste, with and without deproteinisation. Waste was subjected to deproteinisation using alkali and enzyme (pancreatin). The different solvent systems tried were ether:acetone:water (15:75:10 v/v/v), acetone, hexane:isopropanol (3:2 v/v) and 90% acetone v/v. Astaxanthin in the extract was quantified by measuring the OD at 470 nm in hexane. Extraction was also done using vegetable oils viz. coconut oil, soybean oil and sunflower oil. Quantification of astaxanthin in pigmented oil was done by measuring the absorbance at 485 nm using 2155 as extinction coefficient. Astaxanthin yields from deproteinised samples were significantly lower than those from non deproteinised samples. The highest astaxanthin yield of 87.14 ± 4.55μg/g was obtained with non deproteinised wet waste extracted using acetone. The astaxanthin yield was significantly lower when oil was used as the extraction medium. Of the three oils coconut oil gave the highest yield. The results showed that acetone is the best solvent for extracting astaxanthin from shrimp shell waste in wet condition. The astaxanthin content in Aristeus alcocki shell waste is double that of Pandalus borealis shell waste, which is currently used as the commercial source of astaxanthin. The deep sea species Aristeus alcocki can thus be considered as a better source of astaxanthin for commercial exploitation than Pandalus borealis. TLC analysis of the shell waste extract showed that it contains free astaxanthin, astaxanthin monoester and astaxanthin diester in the ratio 1:1:2. GLC identification of the fatty acids esterified with astaxanthin revealed that saturated fatty acids, MUFA and PUFA are in the ratio 5:3:2 in monoester, whereas in diester they are in the ratio 4:3:3. The main fatty acids in monoester and diesters are palmitic acid, oleic acid, stearic acid and PUFAs: DHA and EPA. The in vitro antioxidant activity of the astaxanthin extract showed significant hydroxyl radical scavenging activity, superoxide anion scavenging activity and inhibition of lipid peroxidation. The IC50 values obtained were 56.43 ± 1.06 ng/ml, 27.91 ± 0.54 ng/ml and 26.54 ± 0.42 ng/ml, respectively. The antioxidant activity of astaxanthin from Aristeus alcocki was obtained at nanogram levels. This powerful antioxidant function may be due to the unique molecular structure of astaxanthin and synergistic effect of astaxanthin and PUFAs present in the astaxanthin monoester and diester fractions. The astaxanthin extract from shrimp shell waste significantly reduced carageenan induced paw edema in mice, percentage inhibition being 47.83 and 67.11 percent at astaxanthin concentrations of 0.5 mg/kg body weight and 1.0 mg/kg body weight, respectively. The inhibition of inflammation at 1.0mg/kg body weight was greater than that produced by the standard reference drug diclofenac. Cardioprotective effect of astaxanthin was examined in isoproterenol induced myocardial infarction in rats. Levels of diagnostic marker enzymes, LDH, CPK, GOT, GPT, CK, CK-MB in plasma, lipid peroxides, ascorbic acid, reduced glutathione and the activities of glutathione-dependent antioxidant enzymes GPx, GR, GST and antiperoxidate enzymes CAT, SOD and the membrane bound enzyme Na+ - K+ ATPase in the heart tissues of experimental groups of rats were determined. The prior administration of astaxanthin @ 10mg/kg feed for 45 days significantly prevented the isoproterenol-induced elevation in the levels of diagnostic marker enzymes in plasma, induction of lipid peroxidation and alterations in the level of reduced glutathione and in the activities of glutathione dependent antioxidant enzymes and antiperoxidative enzymes of experimental rats. Feeding astaxanthin caused a decrease in the inhibition of Na+ - K+ ATPase activity against isoproterenol induced myocardial infarction. The powerful cardioprotective effect of astaxanthin can be attributed to the multiple independent mechanisms viz. antioxidant effects, singlet oxygen quenching ability and inhibition of lipid peroxidation of membranes, increased functional gap junctional intercellular communication, anti-inflammatory effects etc. Immunostimulatory action of astaxanthin extract was evaluated in experimental mice. Astaxanthin administration was found to enhance the proliferation of spleen cells and bone marrow cells. Esterase activity was found to be enhanced in bone marrow cells indicating increased maturation of cells of lymophoid linkage. Astaxanthin also enhanced number of antibody forming cells and circulating antibody titre. Thus astaxanthin exhibits strong immunomodulating properties. A significant reduction in the viability of ascites tumour cells DLA in vitro was noted in the current study. The % viability was reduced to 4.34 % at a concentration of 15μg astaxanthin/ml. The cytotoxic action of astaxanthin against DLA may be through induction of apoptosis or through a different pathway. Antitumour activity of astaxanthin was studied by ascite and solid tumour models in mice. An increase in life span of about 67 % was noted in DLA bearing mice administered with astaxanthin at 5 mg/kg body weight. The tumour volume and tumour weight were significantly lower in mice injected with 5 mg/kg body weight astaxanthin. In vitro studies revealed that astaxanthin from shrimp shell waste of Aristeus alcocki inhibited the proliferation of cervical cancer cells HeLa in a dose dependent manner.ThesisItem Open Access Genetic characterization, controlled breeding and development of transgenic varieties of puntius denisonii (day, 1865).(Department of Aquaculture, College of Fisheries, Panangad, 2010) Manoj, C K; KAU; Mohanakumaran Nair, CPuntius denisonii, a beautiful ornamental fish indigenous to the Western Ghats, which has been indiscriminately exploited from the different rivers of Kerala has been recently declared to be vulnerable by the IUCN. The population structure and genetic diversity of P. denisonii has not yet been studied and documented. Many previous attempts to breed this fish in captivity have yielded negative results. The increasing demand for this fish to decorate aquariums worldwide could be satisfied only by developing controlled breeding techniques and larval rearing of its fry. In the present study, the present population structure of P. denisonii has been studied combining both phenotypic and genotypic techniques. Fishes were collected from Irrity, Chaliyar and Periyar rivers of Kerala. Truss network analysis was conducted and the size adjusted morphometric variables were subjected to Principal Component Analysis and Canonical Variance Analysis. Scatter diagram and Dendrogram was plotted using PCA and CVA loadings. The Irrity and the Chaliyar populations were grouped on the positive sector of the PC and CV component showing morphological similarities between the two populations while the Periyar population was placed in the negative sector of the component separated far from the other two. The PC scores were used to find out the variables showing maximum variation between fishes collected from different rivers. RAPD PCR was conducted after isolating DNA from the fins of different populations of P. denisonii. Universal random primers were screened and the primers that produced reproducible bands were selected. Popgene analysis of the binary data yielded the genetic structure of different populations of P. denisonii. Number and percentage of polymorphic loci, Nei's (1973) gene diversity, Shannon's Information index Lewontin (1972), Nei's Unbiased Measures of Genetic Identity and Genetic distance and Dendrogram Based Nei's (1978) Genetic distance using UPGMA --Modified from NEIGHBOR procedure of PHYLIP Version 3.5 were studied. The results obtained supports the truss analysis in that the Irrity and Chaliyar populations in Northern Kerala are genetically more similar while that of Periyar population in Central Kerala are distinct. P. denisonii was successfully induced bred under controlled conditions with synthetic hormone preparations Ovaprim and WOVA-FH. Stress during transport and handling was minimized and live feed was supplemented to enhance maturation of the broodstock. The whole developmental sequence starting from fertilized eggs to hatching was photographed and documented. It took 29-30 hours for the eggs to hatch at 280C. Rearing of fry was successfully accomplished under laboratory conditions. In an attempt to develop transgenic varieties of P. denisonii, pCMV-GFP was electroporated into newly fertilized eggs, maintained in hypoosmolar electroporation buffer. The electroporation parameters that yielded best results were 20V, 3 bursts at 1 second interval. Fin clips were taken from the transgenic individuals reared for a period of 6 weeks. Dot blot test was positive showing integration of the GFP gene in P. denisonii, eventhough expression was not detected under blue or UV light. The genetic and phenotypic data of P. denisonii populations in the present study will aid as a base line for formulating conservation procedures to protect the genetic diversity of wild ones. Stock identification studies are recommended for more concise information on each population. Moreover, the larval rearing and controlled breeding techniques along with the genetic diversity studies will help to design captive breeding programs and enhance the production of hatchery bred ones to meet increasing demand. Further research is recommended for generating transgenic lines with uniform GFP expression.ThesisItem Open Access Enhancement of propagation efficiency in exotic varities of heliconia(Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2008) Reshmi, C R; KAU; Sheela, V LHeliconias are among the most popular garden plants, both for the ease with which they can be grown and the sheer magnificence of the blooms. Studies were conducted to standardize rapid propagation techniques under in vitro and in vivo conditions. Three heliconia varieties belonging to three distinct groups viz., St. Vincent Red (small erect type), Golden Torch Adrian (hybrid) and Sexy Pink (large pendent type) were selected for the study. For in vitro culture establishment, shoot tip explants were effective. The treatment of shoot tips with 0.10 per cent mercuric chloride for ten minutes followed by dipping in 0.05 per cent mercuric chloride for five minutes (after trimming) resulted in better surface sterilization. Longitudinal cutting of the in vitro established shoot tip with apical dome into two halves and culturing them separately produced the highest number of axillary buds. Addition of 0.05 per cent activated charcoal to the establishment media reduced the media browning and hastened shoot initiation. For culture establishment of all the three varieties, MS medium supplemented with BA 5.00 mg l-1 was found to be the best. Murashige and Skoog medium supplemented with BA 2.00 mg l-1 resulted in the highest shoot proliferation in the variety St. Vincent Red. In Golden Torch Adrian, BA 2.00 mg l-1 + NAA 0.20 mg l-1 gave better results. In the variety Sexy Pink, Kinetin 5.00 mg l-1 + NAA 0.20 mg l-1 was the best. For further multiplication in Sexy Pink, BA 1.00 mg l-1 was sufficient. Solid culture medium was better for shoot proliferation in the variety Sexy Pink. Higher sucrose concentration (40.00 g l-1) increased the multiplication rate, but reduced the length of shoots as well as the number of leaves. Addition of activated charcoal to the media as well as exposure of cultures to light had negative effect on shoot proliferation. Full MS medium was found to be the best for in vitro rooting of the variety Sexy Pink. Addition of NAA 0.50 mg l-1 to the MS medium gave better results for in vitro rooting in Golden Torch Adrian and Sexy Pink. Sucrose @ 30.00 g l-1 was sufficient for in vitro rooting in the variety Sexy Pink. Activated charcoal delayed root initiation and reduced the number of roots in the variety Sexy Pink. Sand recorded 90.00 per cent survival in all the three varieties after two months of planting out. At varietal level, significant difference was evident in the total number of suckers. In the first experiment, the variety St. Vincent Red (3.82) was significantly superior in terms of total number of suckers. However, in the second experiment, St. Vincent Red (4.06) was on par with Golden Torch Adrian (4.10). The variety Sexy Pink produced comparatively taller suckers in both the trials. Varietal variation in the number of leaves was observed only in the earlier stages. During the preliminary field experiment, the variety Sexy Pink excelled in leaf area at almost all stages of observation. Among the three varieties, highest collar girth was recorded by suckers of Sexy Pink variety during the first two stages of observation. However, towards the later stages, it was statistically on par with St. Vincent Red. Foliar spray was superior to rhizome dip in terms of total number of suckers, height of suckers and the number of leaves. In the case of leaf area, both the treatments were more or less on par. Application of growth regulators had pronounced effect on sucker production at all the stages during the first experiment when BA 750 mg l-1 produced the highest number (4.19) of total suckers. In the second experiment, variation was evident only in the total number of suckers. Here, BA 850 mg l-1 produced the highest number (4.33) of suckers and it was on par with BA 700 mg l-1 (4.00) and GA3 650 mg l-1 (3.79). Irrespective of the stage of plant growth, gibberellic acid produced taller suckers and BA 500 mg l-1 resulted in the shortest suckers. BA treatments recorded comparatively higher number of leaves. Growth regulator application had remarkably influenced the leaf area of suckers also. Application of BA 1000 mg l-1 (3.53 cm) and GA3 800 mg l-1 (3.33 cm) recorded higher collar girth in suckers. VG interaction exerted significant variation in the number of suckers. At varietal level, BA 750 mg l-1 produced the highest number of suckers in St. Vincent Red (4.75), GA3 500 mg l-1 in Golden Torch Adrian (4.63) and GA3 750 mg l-1 in Sexy Pink (4.00). Among VG treatment combinations in the second experiment, the highest number of suckers (4.75) in the variety St. Vincent Red was produced by BA 700 mg l-1. The varieties Golden Torch Adrian (4.88) and Sexy Pink (3.75) recorded the highest with BA 850 mg l-1. Gibberellic acid produced taller suckers. In the second experiment, VG interaction had no significant effect on the height of suckers. Regarding the number of leaves, BA resulted in comparatively higher number of leaves in both the experiments. In all the three varieties, BA 850 mg l-1 produced the highest number of leaves. Collar girth was found to increase with increase in the concentrations of BA and GA3 in all the three varieties. The economics of foliar application of growth regulators revealed that BA 850 mg l-1 significantly enhanced the profit in the varieties Golden Torch Adrian and Sexy Pink. Although negligible, BA 700 mg l-1 recorded slight positive response in the variety St. Vincent Red with respect to profit.ThesisItem Open Access Women empowerment through group action in the kudumbashree programme of kerala: a multidimensional analysis(Department Of Agricultural Extension, College Of Agriculture, Vellayani, 2007) Priya Devi, R; KAU; Mothilal Nehru, SThe study was aimed to assess the extent of eradication of poverty and the dimensions of empowerment in the Kudumbashree programme of Kerala government for poverty alleviation. The determinants of performance of Self Help Groups organized in neighborhood basis and their functional and structural analysis including the tangible and intangible benefits and constraints were also studied. Suggestions to enhance the efficiency of the Self Help Groups(SHGs) in agriculture and other sectors organized under the Kudumbashree programme were made. A sample of 300 respondents was selected at random from three districts of three regions of the state namely Thiruvananthapuram from south Kerala, Ernakulam from central Kerala and Malappuram from north Kerala which were selected randomly. In each district, 25 SHGs comprising 20 non-agriculture and 5 agriculture oriented SHGs were selected. The structural and functional analysis of Kudumbashree was carried out by Mc Kinsey procedure of SWOT analysis. The implementing category of personnel namely top management, middle management and grass-root functionaries and the selected SHG members were the respondents for the study. The nature, extent and style of functioning of Kudumbashree were studied with the above respondents.Besides, all implementing personnel working in the above three districts were also selected. Interactions with state level management personnel were done to elicit information regarding the study and also for triangulating findings. A sub sample consisting of five poorly performing SHGs from agriculture and non-agriculture sectors making total of ten SHGs on the basis of perception of the implementing officials and the result of the study were selected for in depth sub sample analysis and their limitations were stated. In the study it was assumed that the dependent variables poverty eradication and women empowerment were interrelated with the performance level of the groups having an intervening effect .A measurement scale for PEI was developed to measure extent of poverty eradication which was one of the dependent variable as a specific objective of the study. Poverty eradication index was the yardstick or standard to measure how far the Kudumbashree has alleviated the incidence of poverty among the members .The index consisted of eight constitutive factors and the cumulative expression of the scores in relation to the factors is the poverty eradication index of the respondent. The data was gathered by using the pre tested interview schedule / questionnaire developed for the study. Statistical tools used in the study were quartile deviation, mean, ANOVA and correlation analysis. Poverty eradication index score and empowerment score were given in quartile range to show the extent of poverty eradication and empowerment attained by the Kudumbashree programme. Categorisation was done in low, medium, high and over. As recorded in the study, regarding health care majority of the respondents were dependent on primary health care centre and the constraints felt were lack of supply of medicines, doctors not being available for ensuring timely medical care and the primary health centre located far away from the home. Nature of savings of respondents were with majority having group savings while post office savings, insurance and chitties were also reported.Borrowing pattern regarding the source of borrowing by the respondents showed only reliable institutional sources were used to avail loans by the majority.For purpose of borrowing repayment of debt was recorded as the highest felt need requiring borrowings followed by shelter renovation activities and health care need. Regarding the confidence of repayment majority reported they were confident of repayment while borrowing the amount. But 270 of them had experienced instances of inability to repay on time. 276 respondents of them had felt mental stress due to non repayment of borrowed amount on time. Access to safe drinking water and the source for drinking water as recorded in the study had half of the respondents having access to safe drinking water in their house premises itself. Regarding the vulnerability status of the respondents it was seen that majority of them had faced a situation were they have been unemployed for a month or more. They took loan or lent out assets in the situation. Regarding alcoholism 120 of respondents agreed that the earning male member of the family was alcoholic. Control over earnings had majority satisfied with the expenditure pattern of the family. Regarding entrepreneurial culture 60 of respondents had a temporary job while 99 had permanent job. 75 had reported to go to job daily while 60 had job availability sometimes only. All respondents had reported that they think the work undertaken by them was respectable in society. According to perception of respondents, repayment of loan and not possessing own home were the major factors of poverty while risk factors were damaged house or having no own house. The next constraint was recorded for having no or only one regular employed person in the family. Poverty eradication index ranged from a minimum of 19.51 in all three districts to a maximum of 60.53 in Thiruvananthapuram and 65.76 in both Ernakulam and Malappuram .The mean index was 41.26 in Thiruvananthapuram, 43.92 in Ernakulam and 41.07 in Malappuram. Thus a wide variation in the extent of poverty eradication was shown .The activities will have to be strengthened further to ensure appreciable alleviation of poverty. Empowerment index recorded a minimum of 29.70 in all three districts to a maximum of 86.07 in Thiruvananthapuram and 77.68 in both Ernakulam and Malappuram .The mean index was 55.10 in Thiruvananthapuram, 53.03 in Ernakulam and 51.36 in Malappuram. Regarding relationship between poverty eradication index and empowerment index, all three districts showed significant relationship in this study.Analysis of Variance (ANOVA) test showed significant results for empowerment index. Inferences from categorization of groups in the districts based on group performance index were that medium level of group performance was shown by majority of groups. In depth sub sample analysis had the following limitations identified. For the group doing rice farming, the owners are reluctant to lease out every season and processing units and storage facilities were absent. The members of the computer units had to meet extra expenses of electricity bill and the rent for the building from the income. Delay in payment of works undertaken for public purposes was reported. Marketing is a difficult task for coconut oil and for pappad making units.Drudgery, improper risk management, improper auditing and absence of diversification of crops were also reported. Design of the action program was done with formulation of plan for vegetable cultivation with College of Agriculture,Vellayani as source to get timely information, inputs and technical support.Execution of action program was done and impact assessed by proper monitoring of the process, identifying technological constraints with its solutions. SWOT analysis of Kudumbasree program showed that the localised social upliftment measures by community based organization, enhanced participation through meetings at home and the facilitation of women to enter public life was the major strengths. Productive usage of loan amount through canalised allotment of resources and holistic identification of poor not based on income/consumption but based on risk factors of poverty were the major strengths of the programme. The weaknesses of the programme recorded were leadership being influenced by political interference leading to corruption in work culture and intra group conflicts have been reported to limit the success of certain enterprises especially incase of consumptive usage of credit in absence of proper supervision. Lacks of enthusiasm among young members, cases of late approval of projects or absence of skill development for increasing the market competency were noticed. The opportunities of Kudumbasree program were establishment of social, security network for destitute rehabilitation and to increase the level of participation of members. The preparation of micro plan using the risk indices and problem identification mechanism were also opportunity. Small enterprises can be used for diversification and modernisation of rural economy and skill up gradation of general poor population. The threats include absence of quality control for ensuring market competency, dissipating enthusiasm and lack of proper tie up with line departments .Lack of autonomy in policy formulation lead to micro plan being not given due consideration. Charge officers are not provided technical up gradation for supporting the enterprises and the products lacked local market demand. Tangible and intangible benefits identified in the study were extension of decentralization process to further grass root levels, enhanced female control over earnings, diversification and modernisation of rural economy, sustainable maintenance of Kerala model of development, dependable and trust worthy social network generated, and the reduction in social discrimination and social evils. At the level of implementation of Kudumbasree program benefits were enhanced production lending in the groups than consumption lending, non economic risk indices in identification of the beneficiaries, the multifaceted frame work to alleviate root causes of poverty , reduction in under employment and educated unemployment followed by the provision of separate administration over centrally sponsored programs . The benefits at beneficiary level included constituting a working group for poverty reduction and the habit of savings first and credit later aiding to self dependence. The existence of a group fund gave members experience in money management and trust in people ensure repayment. Also enhanced food security by undertaking rice farming, participatory need based decision making and the ability of NHGs to act as a forum to share concern were other benefits. The major constraints in planning level were panchayaths not creatively participating in project design and micro enterprise planning followed by lack of symbolic policy formulation from part of State Government. Regarding the implementation level the major constraint was recorded as no remuneration paid for the activities undertaken by ADS and CDS Secretaries is followed by the enterprises not being matched with market demands or incompatible to daily routine of members. Mixing up the poor and non- poor in groups was a sure path to failure. Also some beneficiaries sell the productive assets for immediate financial benefit which limits timely repayment. Suggestions on the basis of the study were that rejuvenation and intensification of the Kudumbashree need appraisals and campaigns to limit exploitation in a target oriented manner. Policy formulations from State Government for investment in social capital have to be given more priority; sales promotion must be undertaken widely assisted with innovative enterprise planning and implementation. Technical support for finance management and common supply of raw materials like Bamboos at reasonable cost should be ensured. The stationery supply for canteens can also be organised in common basis by using the wide network generated. The link agents between NHGs and CDS should be ensured proper payment. Sustainability measures including marketing outlets and reduction in drudgery, the schedule for loan recovery specific to enterprise according to the pattern of returns and prevention of sale of assets purchased must be ensured. Proper representation of general problems by consolidation and prioritisation of such ideas to micro plans should be undertaken. More profitable enterprises involving more members by sharing of experiences must be done. Incentives for timely repayment, proper guidance and conflict management cell should be ensured. Suggestions to improve functioning of agriculture oriented groups is to develop a regulated system to pass on specification of the orders to units, timely supply of quality inputs like raw materials through proper channels should be undertaken. Documentation of activities can organize the activities in a scientific manner. Interested members should be motivated by trainings. Small scale units of diverse products with market demand like medicinal plants, vermicomposting and mushroom culture should be organized widely. Proper grading, hygiene and attractive packing to ensure market competency should be done within the local infrastructure available.ThesisItem Open Access Detergent potential of enzymes of dairy microflora and their effect on the shelf life of milk products(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 2010) Beena, A K; KAU; Geevarghese, P IA study was conducted to assess the detergent potential of a spoilage protease enzyme obtained from the microflora of dairy plant environment. An attempt was also made to study the impact of selected enzyme producers on the shelf life of curd (dahi) and sterilised skim milk. A total of 71 bacterial isolates obtained from dairy environment were screened for their ability to produce spoilage enzymes like proteases lipases and lecithinases. Based on the spoilage potential, Pseudomonas aeruginosa (P12) isolated from pasteurised milk and Bacillus cereus (S4) isolated from sterilized skim milk were selected for further work. The influence of spoilage enzymes on selected physico-chemical characteristics of curd (dahi) and sterilized skim milk was evaluated by preparing the products from milk precultured with isolate P12 and S4. In general, proteolysis of milk was found to have an adverse effect on the quality of products. The stimulatory effect of proteolytic products of P12 and S4 on curd starters was evident from the higher values of acidity, firmness and syneresis in treated curd. The spoilage enzymes adversely affected the overall quality and shelf life of curd. In treated sterilised milk, tyrosine and NPN values were highly elevated. A linear correlation was found to exist between off-flavour and proteolysis. Curd and sterilised skim milk prepared from milk precultured with proteolytic organism were significantly different from that of control. The possibility of exploiting an alkaline protease from spoilage organism in dairy plant sanitation was also looked into. Environmental conditions for the production of alkaline protease by a psychrotrophic strain of Bacillus cereus (S4) was optimised in whey based medium. The protease used in this trial preferred an alkaline medium to remain stable. The enzyme was found to be stable over a wide temperature range of -10°C to 80°C and a pH range of 7.0 to 12.0. The metal ions Ca++, Mg++, Zn++ and Hg++ enhanced the enzyme activity. Lack of inhibition by Hg++ suggested lack of disulphide bonds in the active site of enzyme. Significant inhibition of activity by serine inhibitors indicated an essential serine residue in the active site of enzyme. The deleterious effect of EDTA on enzyme activity showed the supportive role of divalent cations. Marked residual activity on treatment with β-mercaptoethanol indicated the absence of cysteine residue for the enzyme. Enhancement of protease activity in the presence of surfactants and stability in the presence of H2O2 signified its potential to be used as detergent additive. Qualitative assessment of cleaning efficiency of inbuilt formulation substantiated the superiority of enzyme based formulations. Ammonium sulphate fractionation, dialysis and gel filtation using seralose 4B and Seralose 6B were effective in purifying the protease preparation by 141.31 fold. The purified protease was found to be a homogenous preparation of molecular weight of 50.5 kDa as determined by SDS PAGE.ThesisItem Open Access Development and evaluation of different vaccines against duck pasteurellosis(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 2010) Jesto, George; KAU; Krishnan NairThis study was undertaken to develop biofilm vaccines against duck pasteurellosis using oil, saponin and aluminium hydroxide as adjuvants and to experimentally evaluate their immunogenicity in ducks. Identity of P. multocida serotype A: 1 (DP1) used for study was confirmed by biochemical tests and by PM-PCR and pathogenicity was established in Swiss albino mice before vaccine production. The LD50 (11 w) of the DP1 isolate determined was 10 CFU / bird and MDT was 23.75 h in 11 week old ducklings when a high dose of 3 X 10 6 CFU of P. multocida per bird was given. At 11 Weeks age MDT gradually increased as the dose of inoculum decreased. In 21 week old ducks, the LD50 (21w) of the isolate DP1 was estimated to be 3 ×108 CFU of P. multocida and it showed that the Kuttanad duck had decreased susceptibility to pasteurellosis with age. On light microscopic studies planktonic cells appeared to be Gram negative coccobacillary, while biofilm cells were Gram negative and pleomorphic. Electron microscopic studies revealed that P. multocida could form well differentiated classic biofilm and 0.32 per cent TSB media supplemented 0.5 per cent chitin seemed to be excellent medium for biofilm formation by P. multocida. Four different vaccines viz. OV, OBV, SV and SAV were prepared and all of them were found to be sterile and safe. The oil adjuvanted vaccines (OV and OBV) offered better protection compared to saponin and aluminium hydroxide adjuvanted vaccine groups (SV and SAV) following primary vaccination, up to seven weeks. The modified oil adjuvanted vaccine prepared was not only found to be homogenous, but also more efficient in stimulating a humoral immune response and hence may be recommended. The oil adjuvanation gave better protection than saponin and aluminium hydroxide adjuvanation. The SAV gave better protection than SV which might be due to the presence of aluminium hydroxide which potentiated the immunostimulating ability of saponin. The combined vaccine (SAV) although was found to be better than single vaccine (SV) they cannot be used as a substitute to oil adjuvanted vaccines. The booster vaccination was found to have added advantageous effect on protection and is a must, to prevent losses. Pasteurella biofilms although found to be weak in inducing a primary immune response had the potency to evoke a more powerful secondary response compared to planktonic cells. Vaccination done at six weeks age followed by booster vaccination at 16 weeks age seemed to be a better modification of existing schedule and may be recommended. In histopathological studies, lymphoid hyperplasia was observed in spleen in survived control birds and in SV and SAV vaccine groups that did not survive challenge test, which indicated the persistence of Pasteurella organisms through mild infection in them following experimental challenge. Lymphoid depletion was observed in caecal tonsil in experimental pasteurellosis as in spleen. As the survived vaccinated birds following challenge test showed normal intact caecal tonsil, the course of disease and lesions might be less prominent in vaccinated birds during infection process. The well developed bursa observed in OV and OBV birds that survived challenge test indicated that the humoral immune response was well induced in them compared to other groups. The designed primers E1 and E2 amplified the gene E and hence, this pair of primers could be used for the production of amplified Gene E sequences for further studies on recombinant ghost system. In conclusion, 0.32 per cent TSB media supplemented 0.5 per cent chitin seemed to be an excellent medium that support classical biofilm formation by P. multocida. Booster vaccination definitely had added advantageous effect on protection. Immunization at 6 weeks of age with OV followed by booster vaccination at 16 weeks age with OBV seemed to be a better modification of existing schedule and may be recommended. In histopathological studies, the lesions were less prominent in vaccinated birds than control birds which indicated that the vaccines were effective.ThesisItem Open Access Sulphur and boron nutrition and their foliar diagnosis in sesame(Department of Soil Science and Agricultural Chemistry,College of Agriculture, Vellayani, 2010) Jeena, Mathew; KAU; Sumam, GeorgeA laboratory cum field experiment was conducted to study the effect of S and B on the growth, yield and quality of sesame var. Thilarani and to standardize the foliar diagnosis of these elements in Onattukara sandy loam soil. The study included an incubation study and two field experiments. The treatments comprising the different levels of S and B laid out in 42factorial RBD. The treatments were T1(S0B0), T2(S0B1), T3(S0B2), T4(S0B3), T5(S1B0), T6(S1B1), T7(S1B2), T8(S1B3), T9(S2B0), T10(S2B1), T11(S2B2), T12(S2B3), T13(S3B0), T14(S3B1), T15(S3B2), T16(S3B3). The different levels of S were S0 (0 kg ha-1), S1 (7.5 kg ha-1), S2, (15 kg ha-1) and S3 (30 kg ha-1) and B0 (0 kg ha-1), B1(2.5 kg ha- 1), B2 (5 kg ha-1) and B3 (7.5 kg ha-1). The incubation study was conducted at College of Agriculture, Vellayani to understand the dissolution and release pattern of S and B from their sources gypsum and borax respectively in Onattukara sandy soil. The results revealed that the release of S and B was maximum at the 30th DOI. Increasing levels of S and B has a positive influence on the S content of the soil. T16 (S3B3) recorded the highest value at all the sampling stages for S whereas in the case of B, the treatment combinations which received B at the highest levels in combination with S3 or S2 showed the highest value. The field experiments were laid out at ORARS, Kayamkulam in 42 factorial RBD having two replications using Thilarani as the test crop. It was observed that application of S and B favourably influenced the yield and yield attributes of sesame. T16 was found to be the treatment which gave the highest grain yield and oil yield in both the years and was found to be on par with T14 (S3B1). S3 was the superior S level. As for the different levels of B, B1 can be inferred as the best level. The content of saturated fatty acids such as palmatic and stearic acid showed a decreasing trend with increasing levels of S and B whereas the content of the unsaturated fatty acids showed an increasing trend. The quality attributes of oil such as acid value, iodine value and saponification value was also studied and it was found that there is a decreasing trend with regard to acid and saponification value and an increasing trend for iodine number. The grain protein content also showed an increasing trend with the increase in rate of application of S and B. Regarding the content and uptake of N, P, K, S, B, Fe, Mn, Cu and Zn, a favourable influence for the different levels of S and B was recorded. Results regarding the S and B use efficiency and their apparent recovery showed that with increase in levels of S, an increasing trend was observed for S. In the case of B, increase was noticed up to B1 (2.5 kg ha-1) and there after showed a decreasing trend. This positive influence was also reflected on the available nutrient status of the soil such as organic carbon content, available N, P, K, S, B and DTPA extractable micronutrients. Correlation studies conducted to standardize the part and stage of sampling for the foliar diagnosis of sesame showed petiole at 30 DAS and 20 DAS in the case of S and B respectively. The same stages were found for the soil sampling also for both the nutrients. The critical nutrient level in the part standardised for these two nutrients were standardized using the graphical method proposed by Cate and Nelson (1965). In the case of S, it had been standardized as 0.088 per cent and for B, it had been found to be 28 mg kg-1. The critical nutrient level in soil was also estimated using the scatter diagram technique and was found to be 23 kg ha-1 at 30 DAS for S and 1.4 ppm at 20 DAS for B. Hence the application of S @ 30 kg ha-1 and B @ 2.5 kg ha-1 could faourably enhance growth of sesame with regard to the growth characters, yield and yield attributes and the quality aspects. Moreover, analysis of the plant and soil samples at the critical stages fixed for the respective nutrients will provide the necessary data for the sustainable management of the crop in Onattukara sandy loam soil.ThesisItem Open Access Population dynamics and management of aphids in vegetable ecosystem(Department of Agricultural Entomology, College of Agriculture, Vellayani, 2009) Thamilvel, D; KAU; Hebsy, BaiEight species of aphids viz., A. gossypii, A. craccivora, A. spiraecola, A. fabae, A. nerii, H. setariae, M. persicae and L. erysimi were recorded from 32 vegetables in a survey conducted in the four taluks of Thiruvananthapuram district of Kerala. Occurrence of A. gossypii on C. grandis, M. oleifera and P. tetragonolobus; A. craccivora on C. gladiata, P. tetragonolobus and S. grandiflora ; A. spiraecola on A. tricolor, C. sativus, C. tetragonoloba, M. oleifera, M. charantia, M. koeingii, P. tetragonolobus and S. androgynus ; M. persicae on A. tricolor and R. sativu; A. nerii on C. annuum and C. frutescens was recorded for the first time from the State and H. setariae on A. tricolor and A. dubius from South India. One hundred and twenty two other plants were identified as host plants of the different aphids. A. gossypii was the dominant species noted, closely followed by A. craccivora, and A. spiraecola. Maximum species of aphids were recorded on amaranthus followed by winged bean. Among the vegetables surveyed, chilli and coccinia were highly susceptible to A. gossypii and winged bean and cowpea to A. craccivora. Mosaic disease incidence was noted in 10 vegetables. Predators were the predominant group of natural enemies associated with the aphids with the coccinellids constituting the major predatory fauna. Twenty species of coccinellids were recorded from different species of aphids on various host plants of which 13 species were new records from Kerala. M. sexmaculatus was the dominant species followed by C. transversalis, S. latemaculatus and C. septempunctata, Four species of syrphids were recorded of which I. scutellaris was predominant. P. yerburiensis and D. aegrota were recorded for the first time from Kerala. Leucopis sp. was the only Chamaemyiid species recorded from the vegetable fields. The chrysopids, C. carneae and A. octopunctata and the hemerobiid, Micromus sp. were the Neuropteran predators of the aphids recorded of which Micromus sp. was dominant. A. octopunctata was recorded for the first time from Kerala. Eleven species of spiders were observed in the vegetable fields among which, O. javanus, T.mandibulata, O. quadridentatus, O. shweta and Phidippus sp. were the frequently encountered species. Aphidius sp. was the most dominant parasitoid in the vegetable ecosystem. Aphelinus sp. and D. rapae were the other parasitoids recorded. Seven species of ants were observed attending the aphids. Studies on the population fluctuation of A. gossypii in chilli and A. craccivora in winged bean during a cropping season indicated that high population was seen during November and December. Correlation studies revealed a significant and positive correlation between the population of both the aphids with the insect predators, spiders and parasitoids. None of the climatic parameters had any significant influence on the aphid population. Excepting, maximum temperature which had a positive influence, all the other weather parameters viz., minimum temperature, relative humidity, rainfall and wind velocity had a negative influence on the population of the predators and parasitoids. Among the botanicals screened in the laboratory, only neem oil- garlic emulsion 2% and NeemAzal T/S 4 ml/l recorded more than 50 per cent mortality of A. gossypii and A. craccivora. Among the insecticides, the neonicotinoids viz., acetamiprid 0.002% imidacloprid, 0.003%, and dimethoate 0.05% proved superior to all other treatments. Dimethoate 0.05% was highly toxic to the coccinellids, syrphids and a hemerobiid (Micromus sp.) predator under laboratory condition. Between the two neonicotinods, acetamiprid 0.002% registered higher mortality than imidacloprid 0.003% whereas NeemAzal T/S 4 ml/l and neem oil + garlic emulsion 2% were safe to the predators. Foliar application of dimethoate 0.05%, acetamiprid 0.002% and imidacloprid 0.003% gave good control of A. gossypii, A. biguttula biguttula, S. dorsalis and A. dispersus in chilli and A. craccivora, A. dispersus, R. pedestris and M. vitrata in winged bean. NeemAzal T/S 4ml/l and neem oil garlic emulsion 2% too checked the population of the pests appreciably. Dimethoate 0.05% was highly toxic to the predators and parasitoids followed by acetamiprid 0.002% both in the chilli and winged bean fields. Comparatively, imidacloprid 0.003% was less toxic. The botanicals were safer to the natural enemies. Dimethoate 0.05% was highly toxic to soil fauna and flora whereas acetamiprid and imidacloprid were less toxic while NeemAzal T/S 4ml/l and neem oil + garlic emulsion 2% were non toxic. In both the trials, significantly higher yield was obtained from all the treatments. However, no significant difference was noted in the yields obtained from acetamiprid 0.002%, imidacloprid 0.003% and dimethoate 0.05% treated plots. Residues of imidacloprid were detected in chilli and winged bean fruits five days after spraying. While on the tenth day after spraying, residues of acetamiprid, and dimethoate were detected in chilli fruits, only residue of acetamiprid was recorded from winged bean pods. Imidacloprid was below detectable level (BDL). Residues of all the three insecticides were below detectable level when estimated fifteen days after spraying. Based on the results of the study, early detection of aphids on weeds and other host through regular monitoring, proper weed and ant management, conservation of the beneficials, application of neem oil + garlic emulsion 2% or NeemAzal T/S 4ml/l during the early stage of infestation and use of imidacloprid 0.003% when needed are suggested for aphid management in vegetables.
