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Kerala Agricultural University, Thrissur
The history of agricultural education in Kerala can be traced back to the year 1896 when a scheme was evolved in the erstwhile Travancore State to train a few young men in scientific agriculture at the Demonstration Farm, Karamana, Thiruvananthapuram, presently, the Cropping Systems Research Centre under Kerala Agricultural University. Agriculture was introduced as an optional subject in the middle school classes in the State in 1922 when an Agricultural Middle School was started at Aluva, Ernakulam District. The popularity and usefulness of this school led to the starting of similar institutions at Kottarakkara and Konni in 1928 and 1931 respectively.
Agriculture was later introduced as an optional subject for Intermediate Course in 1953. In 1955, the erstwhile Government of Travancore-Cochin started the Agricultural College and Research Institute at Vellayani, Thiruvananthapuram and the College of Veterinary and Animal Sciences at Mannuthy, Thrissur for imparting higher education in agricultural and veterinary sciences, respectively. These institutions were brought under the direct administrative control of the Department of Agriculture and the Department of Animal Husbandry, respectively. With the formation of Kerala State in 1956, these two colleges were affiliated to the University of Kerala. The post-graduate programmes leading to M.Sc. (Ag), M.V.Sc. and Ph.D. degrees were started in 1961, 1962 and 1965 respectively.
On the recommendation of the Second National Education Commission (1964-66) headed by Dr. D.S. Kothari, the then Chairman of the University Grants Commission, one Agricultural University in each State was established. The State Agricultural Universities (SAUs) were established in India as an integral part of the National Agricultural Research System to give the much needed impetus to Agriculture Education and Research in the Country. As a result the Kerala Agricultural University (KAU) was established on 24th February 1971 by virtue of the Act 33 of 1971 and started functioning on 1st February 1972. The Kerala Agricultural University is the 15th in the series of the SAUs.
In accordance with the provisions of KAU Act of 1971, the Agricultural College and Research Institute at Vellayani, and the College of Veterinary and Animal Sciences, Mannuthy, were brought under the Kerala Agricultural University. In addition, twenty one agricultural and animal husbandry research stations were also transferred to the KAU for taking up research and extension programmes on various crops, animals, birds, etc.
During 2011, Kerala Agricultural University was trifurcated into Kerala Veterinary and Animal Sciences University (KVASU), Kerala University of Fisheries and Ocean Studies (KUFOS) and Kerala Agricultural University (KAU).
Now the University has seven colleges (four Agriculture, one Agricultural Engineering, one Forestry, one Co-operation Banking & Management), six RARSs, seven KVKs, 15 Research Stations and 16 Research and Extension Units under the faculties of Agriculture, Agricultural Engineering and Forestry. In addition, one Academy on Climate Change Adaptation and one Institute of Agricultural Technology offering M.Sc. (Integrated) Climate Change Adaptation and Diploma in Agricultural Sciences respectively are also functioning in Kerala Agricultural University.
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ThesisItem Open Access Participatory action research for renumerative rice production(Department of Agricultural extension, College of Horticulture, Vellanikkara, 2004) Parvathy, S; KAU; Ahamed, PAs with the Complex, Diverse and Risk- prone (CDR) rice systems of many Asian countries, the Kerala state of peninsular India suffers from the fast depleting paddies and the ' high cost- low remuneration syndrome'. Crucial rice technologies have been blamed by stakeholders for the insensitivity to micro farming situations A multidisciplinary stakeholder Participatory Action Research (PAR) of emancipatory type and collaborative mode was done for three years (2001-2004) on identification and prioritization of constraints to profitable rice production to explore the available cost-reducing and productivity increasing technological options. The project focussed on screening of technological modules through farmer participatory field assessment and arrived at locally adaptable and remunerative technology packages. The project also developed and standardised an extrapolatable stakeholder participatory assessment model and protocol. The programme had a blend of extension approaches, research designs and tools like "ex-post facto", benchmark appraisal through Participatory Learning and Action (PLA), exploratory, diagnostic, evaluative, field experiments and analytical studies. The PAR was done in two rice ecosystems (irrigated and rainfe:l) of the midland, laterite belt of Kerala state, India. Fourteen technology modules were fitted into the PAR, under .. , each of the three treatments, viz., farmers' practice, recommended packages of the formal research system and location specific I technology components" jointly decided by the research team extensionists and farmers. Each technology component was subjected to five types of analysis viz., agronomic, statistical, economic, farmers perceptions and reactions, post-trial follow up analysis of adoption in the succeeding cropping seasons. The participatory interventions significantly influenced the level of technical knowledge and extent of adoption of adaptable technology modules, typifying the cognitive impact of an emancipating action research. The short duration red rice varieties "Kanchana" (Ptb 50) and "Kairali" (Ptb 49) proved to be the best first crop and second crop varieties respectively, to replace the ruling cultivars. The technology modules recommended by the formal research system viz., seed treatment with fungicides for the first crop season and Pseudomonas fluorescence for the second crop season were adaptable technologies. The PAR came out with an efficient planting density and crop geometry package (line transplanting; 15 x 10 cm; 67 . , hills/m'; 2-3 seedlings/hill) to replace the conventional planting system. The existing formal recommendations including IPM and INM practices could enhance and combat weeds, pest and diseases thereby enhancing crop yield. Harvesting with self- propelled reaper and threshing with mechanised thresher were cost effective, drudgery alleviating and time saving. The net result of the action research was a set of adaptable technological package for remunerative rice production in the CDR rice production systems. Cognitive and behavioural impact on the participants; and the standard methodology and protocol for participatory technology validation for rice in particular, and for any farm enterprise in general, with extrapolative effect.ThesisItem Open Access Enhancement of propagation efficiency in exotic varities of heliconia(Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2008) Reshmi, C R; KAU; Sheela, V LHeliconias are among the most popular garden plants, both for the ease with which they can be grown and the sheer magnificence of the blooms. Studies were conducted to standardize rapid propagation techniques under in vitro and in vivo conditions. Three heliconia varieties belonging to three distinct groups viz., St. Vincent Red (small erect type), Golden Torch Adrian (hybrid) and Sexy Pink (large pendent type) were selected for the study. For in vitro culture establishment, shoot tip explants were effective. The treatment of shoot tips with 0.10 per cent mercuric chloride for ten minutes followed by dipping in 0.05 per cent mercuric chloride for five minutes (after trimming) resulted in better surface sterilization. Longitudinal cutting of the in vitro established shoot tip with apical dome into two halves and culturing them separately produced the highest number of axillary buds. Addition of 0.05 per cent activated charcoal to the establishment media reduced the media browning and hastened shoot initiation. For culture establishment of all the three varieties, MS medium supplemented with BA 5.00 mg l-1 was found to be the best. Murashige and Skoog medium supplemented with BA 2.00 mg l-1 resulted in the highest shoot proliferation in the variety St. Vincent Red. In Golden Torch Adrian, BA 2.00 mg l-1 + NAA 0.20 mg l-1 gave better results. In the variety Sexy Pink, Kinetin 5.00 mg l-1 + NAA 0.20 mg l-1 was the best. For further multiplication in Sexy Pink, BA 1.00 mg l-1 was sufficient. Solid culture medium was better for shoot proliferation in the variety Sexy Pink. Higher sucrose concentration (40.00 g l-1) increased the multiplication rate, but reduced the length of shoots as well as the number of leaves. Addition of activated charcoal to the media as well as exposure of cultures to light had negative effect on shoot proliferation. Full MS medium was found to be the best for in vitro rooting of the variety Sexy Pink. Addition of NAA 0.50 mg l-1 to the MS medium gave better results for in vitro rooting in Golden Torch Adrian and Sexy Pink. Sucrose @ 30.00 g l-1 was sufficient for in vitro rooting in the variety Sexy Pink. Activated charcoal delayed root initiation and reduced the number of roots in the variety Sexy Pink. Sand recorded 90.00 per cent survival in all the three varieties after two months of planting out. At varietal level, significant difference was evident in the total number of suckers. In the first experiment, the variety St. Vincent Red (3.82) was significantly superior in terms of total number of suckers. However, in the second experiment, St. Vincent Red (4.06) was on par with Golden Torch Adrian (4.10). The variety Sexy Pink produced comparatively taller suckers in both the trials. Varietal variation in the number of leaves was observed only in the earlier stages. During the preliminary field experiment, the variety Sexy Pink excelled in leaf area at almost all stages of observation. Among the three varieties, highest collar girth was recorded by suckers of Sexy Pink variety during the first two stages of observation. However, towards the later stages, it was statistically on par with St. Vincent Red. Foliar spray was superior to rhizome dip in terms of total number of suckers, height of suckers and the number of leaves. In the case of leaf area, both the treatments were more or less on par. Application of growth regulators had pronounced effect on sucker production at all the stages during the first experiment when BA 750 mg l-1 produced the highest number (4.19) of total suckers. In the second experiment, variation was evident only in the total number of suckers. Here, BA 850 mg l-1 produced the highest number (4.33) of suckers and it was on par with BA 700 mg l-1 (4.00) and GA3 650 mg l-1 (3.79). Irrespective of the stage of plant growth, gibberellic acid produced taller suckers and BA 500 mg l-1 resulted in the shortest suckers. BA treatments recorded comparatively higher number of leaves. Growth regulator application had remarkably influenced the leaf area of suckers also. Application of BA 1000 mg l-1 (3.53 cm) and GA3 800 mg l-1 (3.33 cm) recorded higher collar girth in suckers. VG interaction exerted significant variation in the number of suckers. At varietal level, BA 750 mg l-1 produced the highest number of suckers in St. Vincent Red (4.75), GA3 500 mg l-1 in Golden Torch Adrian (4.63) and GA3 750 mg l-1 in Sexy Pink (4.00). Among VG treatment combinations in the second experiment, the highest number of suckers (4.75) in the variety St. Vincent Red was produced by BA 700 mg l-1. The varieties Golden Torch Adrian (4.88) and Sexy Pink (3.75) recorded the highest with BA 850 mg l-1. Gibberellic acid produced taller suckers. In the second experiment, VG interaction had no significant effect on the height of suckers. Regarding the number of leaves, BA resulted in comparatively higher number of leaves in both the experiments. In all the three varieties, BA 850 mg l-1 produced the highest number of leaves. Collar girth was found to increase with increase in the concentrations of BA and GA3 in all the three varieties. The economics of foliar application of growth regulators revealed that BA 850 mg l-1 significantly enhanced the profit in the varieties Golden Torch Adrian and Sexy Pink. Although negligible, BA 700 mg l-1 recorded slight positive response in the variety St. Vincent Red with respect to profit.ThesisItem Open Access Women empowerment through group action in the kudumbashree programme of kerala: a multidimensional analysis(Department Of Agricultural Extension, College Of Agriculture, Vellayani, 2007) Priya Devi, R; KAU; Mothilal Nehru, SThe study was aimed to assess the extent of eradication of poverty and the dimensions of empowerment in the Kudumbashree programme of Kerala government for poverty alleviation. The determinants of performance of Self Help Groups organized in neighborhood basis and their functional and structural analysis including the tangible and intangible benefits and constraints were also studied. Suggestions to enhance the efficiency of the Self Help Groups(SHGs) in agriculture and other sectors organized under the Kudumbashree programme were made. A sample of 300 respondents was selected at random from three districts of three regions of the state namely Thiruvananthapuram from south Kerala, Ernakulam from central Kerala and Malappuram from north Kerala which were selected randomly. In each district, 25 SHGs comprising 20 non-agriculture and 5 agriculture oriented SHGs were selected. The structural and functional analysis of Kudumbashree was carried out by Mc Kinsey procedure of SWOT analysis. The implementing category of personnel namely top management, middle management and grass-root functionaries and the selected SHG members were the respondents for the study. The nature, extent and style of functioning of Kudumbashree were studied with the above respondents.Besides, all implementing personnel working in the above three districts were also selected. Interactions with state level management personnel were done to elicit information regarding the study and also for triangulating findings. A sub sample consisting of five poorly performing SHGs from agriculture and non-agriculture sectors making total of ten SHGs on the basis of perception of the implementing officials and the result of the study were selected for in depth sub sample analysis and their limitations were stated. In the study it was assumed that the dependent variables poverty eradication and women empowerment were interrelated with the performance level of the groups having an intervening effect .A measurement scale for PEI was developed to measure extent of poverty eradication which was one of the dependent variable as a specific objective of the study. Poverty eradication index was the yardstick or standard to measure how far the Kudumbashree has alleviated the incidence of poverty among the members .The index consisted of eight constitutive factors and the cumulative expression of the scores in relation to the factors is the poverty eradication index of the respondent. The data was gathered by using the pre tested interview schedule / questionnaire developed for the study. Statistical tools used in the study were quartile deviation, mean, ANOVA and correlation analysis. Poverty eradication index score and empowerment score were given in quartile range to show the extent of poverty eradication and empowerment attained by the Kudumbashree programme. Categorisation was done in low, medium, high and over. As recorded in the study, regarding health care majority of the respondents were dependent on primary health care centre and the constraints felt were lack of supply of medicines, doctors not being available for ensuring timely medical care and the primary health centre located far away from the home. Nature of savings of respondents were with majority having group savings while post office savings, insurance and chitties were also reported.Borrowing pattern regarding the source of borrowing by the respondents showed only reliable institutional sources were used to avail loans by the majority.For purpose of borrowing repayment of debt was recorded as the highest felt need requiring borrowings followed by shelter renovation activities and health care need. Regarding the confidence of repayment majority reported they were confident of repayment while borrowing the amount. But 270 of them had experienced instances of inability to repay on time. 276 respondents of them had felt mental stress due to non repayment of borrowed amount on time. Access to safe drinking water and the source for drinking water as recorded in the study had half of the respondents having access to safe drinking water in their house premises itself. Regarding the vulnerability status of the respondents it was seen that majority of them had faced a situation were they have been unemployed for a month or more. They took loan or lent out assets in the situation. Regarding alcoholism 120 of respondents agreed that the earning male member of the family was alcoholic. Control over earnings had majority satisfied with the expenditure pattern of the family. Regarding entrepreneurial culture 60 of respondents had a temporary job while 99 had permanent job. 75 had reported to go to job daily while 60 had job availability sometimes only. All respondents had reported that they think the work undertaken by them was respectable in society. According to perception of respondents, repayment of loan and not possessing own home were the major factors of poverty while risk factors were damaged house or having no own house. The next constraint was recorded for having no or only one regular employed person in the family. Poverty eradication index ranged from a minimum of 19.51 in all three districts to a maximum of 60.53 in Thiruvananthapuram and 65.76 in both Ernakulam and Malappuram .The mean index was 41.26 in Thiruvananthapuram, 43.92 in Ernakulam and 41.07 in Malappuram. Thus a wide variation in the extent of poverty eradication was shown .The activities will have to be strengthened further to ensure appreciable alleviation of poverty. Empowerment index recorded a minimum of 29.70 in all three districts to a maximum of 86.07 in Thiruvananthapuram and 77.68 in both Ernakulam and Malappuram .The mean index was 55.10 in Thiruvananthapuram, 53.03 in Ernakulam and 51.36 in Malappuram. Regarding relationship between poverty eradication index and empowerment index, all three districts showed significant relationship in this study.Analysis of Variance (ANOVA) test showed significant results for empowerment index. Inferences from categorization of groups in the districts based on group performance index were that medium level of group performance was shown by majority of groups. In depth sub sample analysis had the following limitations identified. For the group doing rice farming, the owners are reluctant to lease out every season and processing units and storage facilities were absent. The members of the computer units had to meet extra expenses of electricity bill and the rent for the building from the income. Delay in payment of works undertaken for public purposes was reported. Marketing is a difficult task for coconut oil and for pappad making units.Drudgery, improper risk management, improper auditing and absence of diversification of crops were also reported. Design of the action program was done with formulation of plan for vegetable cultivation with College of Agriculture,Vellayani as source to get timely information, inputs and technical support.Execution of action program was done and impact assessed by proper monitoring of the process, identifying technological constraints with its solutions. SWOT analysis of Kudumbasree program showed that the localised social upliftment measures by community based organization, enhanced participation through meetings at home and the facilitation of women to enter public life was the major strengths. Productive usage of loan amount through canalised allotment of resources and holistic identification of poor not based on income/consumption but based on risk factors of poverty were the major strengths of the programme. The weaknesses of the programme recorded were leadership being influenced by political interference leading to corruption in work culture and intra group conflicts have been reported to limit the success of certain enterprises especially incase of consumptive usage of credit in absence of proper supervision. Lacks of enthusiasm among young members, cases of late approval of projects or absence of skill development for increasing the market competency were noticed. The opportunities of Kudumbasree program were establishment of social, security network for destitute rehabilitation and to increase the level of participation of members. The preparation of micro plan using the risk indices and problem identification mechanism were also opportunity. Small enterprises can be used for diversification and modernisation of rural economy and skill up gradation of general poor population. The threats include absence of quality control for ensuring market competency, dissipating enthusiasm and lack of proper tie up with line departments .Lack of autonomy in policy formulation lead to micro plan being not given due consideration. Charge officers are not provided technical up gradation for supporting the enterprises and the products lacked local market demand. Tangible and intangible benefits identified in the study were extension of decentralization process to further grass root levels, enhanced female control over earnings, diversification and modernisation of rural economy, sustainable maintenance of Kerala model of development, dependable and trust worthy social network generated, and the reduction in social discrimination and social evils. At the level of implementation of Kudumbasree program benefits were enhanced production lending in the groups than consumption lending, non economic risk indices in identification of the beneficiaries, the multifaceted frame work to alleviate root causes of poverty , reduction in under employment and educated unemployment followed by the provision of separate administration over centrally sponsored programs . The benefits at beneficiary level included constituting a working group for poverty reduction and the habit of savings first and credit later aiding to self dependence. The existence of a group fund gave members experience in money management and trust in people ensure repayment. Also enhanced food security by undertaking rice farming, participatory need based decision making and the ability of NHGs to act as a forum to share concern were other benefits. The major constraints in planning level were panchayaths not creatively participating in project design and micro enterprise planning followed by lack of symbolic policy formulation from part of State Government. Regarding the implementation level the major constraint was recorded as no remuneration paid for the activities undertaken by ADS and CDS Secretaries is followed by the enterprises not being matched with market demands or incompatible to daily routine of members. Mixing up the poor and non- poor in groups was a sure path to failure. Also some beneficiaries sell the productive assets for immediate financial benefit which limits timely repayment. Suggestions on the basis of the study were that rejuvenation and intensification of the Kudumbashree need appraisals and campaigns to limit exploitation in a target oriented manner. Policy formulations from State Government for investment in social capital have to be given more priority; sales promotion must be undertaken widely assisted with innovative enterprise planning and implementation. Technical support for finance management and common supply of raw materials like Bamboos at reasonable cost should be ensured. The stationery supply for canteens can also be organised in common basis by using the wide network generated. The link agents between NHGs and CDS should be ensured proper payment. Sustainability measures including marketing outlets and reduction in drudgery, the schedule for loan recovery specific to enterprise according to the pattern of returns and prevention of sale of assets purchased must be ensured. Proper representation of general problems by consolidation and prioritisation of such ideas to micro plans should be undertaken. More profitable enterprises involving more members by sharing of experiences must be done. Incentives for timely repayment, proper guidance and conflict management cell should be ensured. Suggestions to improve functioning of agriculture oriented groups is to develop a regulated system to pass on specification of the orders to units, timely supply of quality inputs like raw materials through proper channels should be undertaken. Documentation of activities can organize the activities in a scientific manner. Interested members should be motivated by trainings. Small scale units of diverse products with market demand like medicinal plants, vermicomposting and mushroom culture should be organized widely. Proper grading, hygiene and attractive packing to ensure market competency should be done within the local infrastructure available.ThesisItem Open Access Population dynamics and management of aphids in vegetable ecosystem(Department of Agricultural Entomology, College of Agriculture, Vellayani, 2009) Thamilvel, D; KAU; Hebsy, BaiEight species of aphids viz., A. gossypii, A. craccivora, A. spiraecola, A. fabae, A. nerii, H. setariae, M. persicae and L. erysimi were recorded from 32 vegetables in a survey conducted in the four taluks of Thiruvananthapuram district of Kerala. Occurrence of A. gossypii on C. grandis, M. oleifera and P. tetragonolobus; A. craccivora on C. gladiata, P. tetragonolobus and S. grandiflora ; A. spiraecola on A. tricolor, C. sativus, C. tetragonoloba, M. oleifera, M. charantia, M. koeingii, P. tetragonolobus and S. androgynus ; M. persicae on A. tricolor and R. sativu; A. nerii on C. annuum and C. frutescens was recorded for the first time from the State and H. setariae on A. tricolor and A. dubius from South India. One hundred and twenty two other plants were identified as host plants of the different aphids. A. gossypii was the dominant species noted, closely followed by A. craccivora, and A. spiraecola. Maximum species of aphids were recorded on amaranthus followed by winged bean. Among the vegetables surveyed, chilli and coccinia were highly susceptible to A. gossypii and winged bean and cowpea to A. craccivora. Mosaic disease incidence was noted in 10 vegetables. Predators were the predominant group of natural enemies associated with the aphids with the coccinellids constituting the major predatory fauna. Twenty species of coccinellids were recorded from different species of aphids on various host plants of which 13 species were new records from Kerala. M. sexmaculatus was the dominant species followed by C. transversalis, S. latemaculatus and C. septempunctata, Four species of syrphids were recorded of which I. scutellaris was predominant. P. yerburiensis and D. aegrota were recorded for the first time from Kerala. Leucopis sp. was the only Chamaemyiid species recorded from the vegetable fields. The chrysopids, C. carneae and A. octopunctata and the hemerobiid, Micromus sp. were the Neuropteran predators of the aphids recorded of which Micromus sp. was dominant. A. octopunctata was recorded for the first time from Kerala. Eleven species of spiders were observed in the vegetable fields among which, O. javanus, T.mandibulata, O. quadridentatus, O. shweta and Phidippus sp. were the frequently encountered species. Aphidius sp. was the most dominant parasitoid in the vegetable ecosystem. Aphelinus sp. and D. rapae were the other parasitoids recorded. Seven species of ants were observed attending the aphids. Studies on the population fluctuation of A. gossypii in chilli and A. craccivora in winged bean during a cropping season indicated that high population was seen during November and December. Correlation studies revealed a significant and positive correlation between the population of both the aphids with the insect predators, spiders and parasitoids. None of the climatic parameters had any significant influence on the aphid population. Excepting, maximum temperature which had a positive influence, all the other weather parameters viz., minimum temperature, relative humidity, rainfall and wind velocity had a negative influence on the population of the predators and parasitoids. Among the botanicals screened in the laboratory, only neem oil- garlic emulsion 2% and NeemAzal T/S 4 ml/l recorded more than 50 per cent mortality of A. gossypii and A. craccivora. Among the insecticides, the neonicotinoids viz., acetamiprid 0.002% imidacloprid, 0.003%, and dimethoate 0.05% proved superior to all other treatments. Dimethoate 0.05% was highly toxic to the coccinellids, syrphids and a hemerobiid (Micromus sp.) predator under laboratory condition. Between the two neonicotinods, acetamiprid 0.002% registered higher mortality than imidacloprid 0.003% whereas NeemAzal T/S 4 ml/l and neem oil + garlic emulsion 2% were safe to the predators. Foliar application of dimethoate 0.05%, acetamiprid 0.002% and imidacloprid 0.003% gave good control of A. gossypii, A. biguttula biguttula, S. dorsalis and A. dispersus in chilli and A. craccivora, A. dispersus, R. pedestris and M. vitrata in winged bean. NeemAzal T/S 4ml/l and neem oil garlic emulsion 2% too checked the population of the pests appreciably. Dimethoate 0.05% was highly toxic to the predators and parasitoids followed by acetamiprid 0.002% both in the chilli and winged bean fields. Comparatively, imidacloprid 0.003% was less toxic. The botanicals were safer to the natural enemies. Dimethoate 0.05% was highly toxic to soil fauna and flora whereas acetamiprid and imidacloprid were less toxic while NeemAzal T/S 4ml/l and neem oil + garlic emulsion 2% were non toxic. In both the trials, significantly higher yield was obtained from all the treatments. However, no significant difference was noted in the yields obtained from acetamiprid 0.002%, imidacloprid 0.003% and dimethoate 0.05% treated plots. Residues of imidacloprid were detected in chilli and winged bean fruits five days after spraying. While on the tenth day after spraying, residues of acetamiprid, and dimethoate were detected in chilli fruits, only residue of acetamiprid was recorded from winged bean pods. Imidacloprid was below detectable level (BDL). Residues of all the three insecticides were below detectable level when estimated fifteen days after spraying. Based on the results of the study, early detection of aphids on weeds and other host through regular monitoring, proper weed and ant management, conservation of the beneficials, application of neem oil + garlic emulsion 2% or NeemAzal T/S 4ml/l during the early stage of infestation and use of imidacloprid 0.003% when needed are suggested for aphid management in vegetables.ThesisItem Open Access Effective agricultural information delivery system-an action research among farmers(Department Of Agricultural Extension, College Of Agriculture, Vellayani, 2008) Nazreen Hassan, S; KAU; Kishore Kumar, NExtension providers and client expectations include superior information accessing and dissemination, ongoing farmer-extension-research communications and marketing. AgIDS offer tremendous potential for accelerating development. They are transforming our lives, creating wealth and impacting every facet of human endeavor. Agricultural marketing requires connectivity between the market and exporters, growers, traders, industry consumers, through wide area network of national and international linkages in order to provide day-to-day information with regard to commodity arrivals and prevailing rates etc. this study was carried out with the objective of bringing out the underlying facts in the Information Communication technology available to the benefit of the farmers. Thiruvananthapuram district has been purposively selected for the study because this is the only district in Kerala having information kiosks being operated in the Krishibhavans. From the ten Krishibhavans three Krishibhavans were selected based on the maximum numbers of users of this ICT facility based on the records available in the Krishibhavans. The respondents were selected by random sampling method. 60 user farmers and 30 non-user farmers from each panchayat were selected and they formed the respondents of this study. A total of 270 farmers were contacted for the study. From a review of related literature and discussion with experts a comprehensive list of variables were selected. The dependent variables included attributes of ICT, Communication behaviour, information need and skill requirement. Majority of the farmers came under middle age group. Majority of the farmer in both the categories were found to be marginal farmers. Mostly the users were in a better socio economic group. The Extension Service must be able to provide information that makes a difference. ICTs are tools that help build human network, increase public awareness and provide access to information and knowledge for the use of people. They include Telephone, Fax, GIS, Radio, Television, Print, Internet, Video, Audio, Computer and technologies on the drawing boards. Possession of ICT tools by users was found to be higher than the nonusers except for farm magazines where the nonusers out-numbered the users. The mean of the socio technical variables was found to be the maximum for physical compatibility and desirability in the case of users. This was followed by social acceptability and simplicity. This was followed by physical compatibility and social acceptability. In both the cases user education had the minimum score. So this area needs more attention. Majority of farmers in both the categories perceived efficiency of ICT as ‘saving time and money in interacting’ and has ‘all information the farmer wants’ in the nonuser category. This was followed by one stop shop information by the users and supports marketing in the case of non users. There existed significant relation ship between the dependent variables. Also significant relationship was observed in the relationship that existed between some independent variables and dependent variables. A majority of the users claim that they slightly agree to the fact that their productivity has increased compared to the previous year. Analysis of variance showed a comparison between the user group and the non user group. It was found that the user group was higher in the case of all dependent variables. But in the case of information need it was found to be almost similar. A need assessment can help Extension educators to decide whether an extension program should be improved and how the services should be better delivered. The farmers needed information on agriculture such as market rates, bio pesticides, bio fertilizers. These areas ranked top in their information needs. This was followed by organic farming, plant protection and cropping practices. The skill requirement of the non-users farmers was more than the user farmers. The action research was conducted in the form of training on the skills of using computers. The participants for the research were selected by random sampling from the list of non user farmers’ interviewed. The farmers were exposed to the skills for five hours for two days i.e., for ten hours each to a group of thirty members. The influence of Training on the non-users observance on their knowledge gain, and skill acquisition at different intervals of time on the existing ICT facility was studied by documenting the knowledge gain, and skill acquisition at one week, one month and three months interval by the trainees. The results of paired‘t’ test on knowledge gain and skill acquisition showed high significance between different intervals of time. As perceived by the farmers it was found that lack in skill was the major problem that prevented most farmers in accessing through the ICT facility. The farmers also gave out some suggestions to bring this facility in a more effective manner. The study indicated anew strategy for solving the problems in technology dissemination by finding out the actual needs of the farmers in harnessing the benefits of ICT.ThesisItem Open Access Intra and inter generic hybridization and molecular charatrization in monopodial orchids(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2008) Beena, Thomas; KAU; Lekha Rani, CA research programme entitled “Intra and inter generic hybridization and molecular characterization in monopodial orchids” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2005-2008 with the objective of undertaking intra and intergeneric hybridization, in vitro embryo culture and molecular characterization in monopodial orchids, as a preliminary step to develop novel hybrids. Fifteen monopodial orchid genotypes comprising of six genera viz., Aranda, Aranthera, Kagawara, Mokara, Renanthera and Vanda, with good cut flower qualities and high demand in the market were selected as parents after initial evaluation. They were evaluated adopting completely randomized design with five replications. Analysis of variance revealed significant differences for almost all the characters studied. Genotypic and phenotypic coefficients of variation were high for thickness of leaf, leaf area and number of aerial roots. High heritability (>70 %) combined with high genetic advance (>70 %) was observed for number of aerial roots, width of leaf, thickness of leaf, leaf area, number of spikes per shoot and number of flowers per inflorescence. Significant positive inter-correlation at genotypic and phenotypic levels was observed for length of flower and width of flower with number of spikes per shoot. The character number of spikes per shoot recorded significant positive correlation with leaf area. Number of flowers per inflorescence was positively correlated with number of leaves per shoot and length of inflorescence. Out of the six genera studied, four viz., Aranda, Aranthera, Kagawara and Mokara, exhibited free-flowering nature. Seasonal flowering was observed mainly from June to December in Vanda, whereas it was confined to two seasons, from February to March and August to October in Renanthera. Inflorescence axis was found to be arching in Aranthera and Renanthera while the rest of the genotypes produced erect inflorescence axis. The 15 parental genotypes were crossed in all possible combinations after preliminary studies on floral biology. A total of 225 cross combinations were attempted including 105 crosses, 105 reciprocals and 15 selfs. Incompatibility reactions were noticed at different stages ranging from flower abscission before the onset of any visible post pollination change to instances where seeds germinated but aborted in culture. Mature green capsules were harvested from 70 combinations at 70 to 90 per cent maturity. Among them 15 combinations did not yield any seeds in the capsule while the remaining 55 combinations were cultured axenically. Among the 55 combinations inoculated in vitro, no germination was obtained from seeds of 12 combinations. Out of the 43 combinations that germinated successfully, seven combinations showed arrested development. Thus out of the total 55 combinations inoculated in vitro 36 combinations developed successfully. These were subcultured three to four times. Seedlings having 2-3 leaves and 2-3 roots were deflasked and planted out. MS half strength was selected as the best basal medium. For improving the in vitro growth of hybrid monopodial orchid seedlings refinement of this medium by supplementing with IAA (8 mg l-1) and NAA (2 mg l-1) was beneficial. Significant differences among the combinations were observed with respect to number of days taken for germination initiation, number of days taken for development of protocorms, chlorophyll, first leaf, first shoot and first root primordia and for deflasking. Significant differences in seedling morphology were observed among the 36 hybrid combinations at deflasking. These were kept in humidity chamber for acclimatization for one month, transferred to net house for hardening and maintained there for further growth. In the present study, RAPD was employed for studying the genetic diversity and for the fingerprinting of 20 monopodial orchid hybrids, making use of arbitrary primers to amplify random DNA sequences in the genome. To identify the promising primers for RAPD analysis, 70 decamer primers of kit A, B, C and D were screened using the DNA of hybrid H-2. Based on the performance in DNA amplification, eight decamer primers were identified for RAPD analysis. Primers that produced highest number of polymorphic bands which were intense and reproducible were selected. They were OPB-07, OPB-15, OPB-l7, OPC-04, OPC-05, OPC-08, OPC-15 and OPD-02. a total of 57 scorable bands (average of 7.125 bands per primer) were generated by the selected eight primers of which six were monomorphic and the remaining 51 were polymorphic (89.47%). The estimation of Jaccard’s coefficients and construction of dendrogram by using UPGMA revealed the presence and extent of genetic similarities among the 20 monopodial orchid hybrids. The overall similarity coefficients ranged from 0.40 to 0.84. Cluster analysis revealed that at 0.69 similarity coefficient, the 20 monopodial orchid hybrids got divided into six groups. Among the 20 hybrids, H-18, H-7, H-13, H-17, H-19 and H-20 stood separately in clusters II, III A, III B, IV, V and cluster VI respectively. This substantiates the moderately broad distribution of genetic variability, which can be attributed to the broad genetic base in their ancestry. Cluster I A contained four hybrids viz., H-1, H-10, H-3 and H-9. This grouping is justified by the presence of a common parent i.e., Arachnis Maggie Oei Red Ribbon in their parentage. Moreover, two common species viz., Arachnis hookeriana and Arachnis flos-aeris are involved in their ancestry. All these support their belonging to the same cluster. The three hybrids such as H-5, H-6 and H-8 fell in cluster I B. RAPD technique is relatively simpler, quicker, less expensive and non-radioactive than other molecular characterization techniques. The results of present investigation proved that it can detect sufficient polymorphisms in genetic distance studies in monopodial orchids. A research programme entitled “Intra and inter generic hybridization and molecular characterization in monopodial orchids” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2005-2008 with the objective of undertaking intra and intergeneric hybridization, in vitro embryo culture and molecular characterization in monopodial orchids, as a preliminary step to develop novel hybrids. Fifteen monopodial orchid genotypes comprising of six genera viz., Aranda, Aranthera, Kagawara, Mokara, Renanthera and Vanda, with good cut flower qualities and high demand in the market were selected as parents after initial evaluation. They were evaluated adopting completely randomized design with five replications. Analysis of variance revealed significant differences for almost all the characters studied. Genotypic and phenotypic coefficients of variation were high for thickness of leaf, leaf area and number of aerial roots. High heritability (>70 %) combined with high genetic advance (>70 %) was observed for number of aerial roots, width of leaf, thickness of leaf, leaf area, number of spikes per shoot and number of flowers per inflorescence. Significant positive inter-correlation at genotypic and phenotypic levels was observed for length of flower and width of flower with number of spikes per shoot. The character number of spikes per shoot recorded significant positive correlation with leaf area. Number of flowers per inflorescence was positively correlated with number of leaves per shoot and length of inflorescence. Out of the six genera studied, four viz., Aranda, Aranthera, Kagawara and Mokara, exhibited free-flowering nature. Seasonal flowering was observed mainly from June to December in Vanda, whereas it was confined to two seasons, from February to March and August to October in Renanthera. Inflorescence axis was found to be arching in Aranthera and Renanthera while the rest of the genotypes produced erect inflorescence axis. The 15 parental genotypes were crossed in all possible combinations after preliminary studies on floral biology. A total of 225 cross combinations were attempted including 105 crosses, 105 reciprocals and 15 selfs. Incompatibility reactions were noticed at different stages ranging from flower abscission before the onset of any visible post pollination change to instances where seeds germinated but aborted in culture. Mature green capsules were harvested from 70 combinations at 70 to 90 per cent maturity. Among them 15 combinations did not yield any seeds in the capsule while the remaining 55 combinations were cultured axenically. Among the 55 combinations inoculated in vitro, no germination was obtained from seeds of 12 combinations. Out of the 43 combinations that germinated successfully, seven combinations showed arrested development. Thus out of the total 55 combinations inoculated in vitro 36 combinations developed successfully. These were subcultured three to four times. Seedlings having 2-3 leaves and 2-3 roots were deflasked and planted out. MS half strength was selected as the best basal medium. For improving the in vitro growth of hybrid monopodial orchid seedlings refinement of this medium by supplementing with IAA (8 mg l-1) and NAA (2 mg l-1) was beneficial. Significant differences among the combinations were observed with respect to number of days taken for germination initiation, number of days taken for development of protocorms, chlorophyll, first leaf, first shoot and first root primordia and for deflasking. Significant differences in seedling morphology were observed among the 36 hybrid combinations at deflasking. These were kept in humidity chamber for acclimatization for one month, transferred to net house for hardening and maintained there for further growth. In the present study, RAPD was employed for studying the genetic diversity and for the fingerprinting of 20 monopodial orchid hybrids, making use of arbitrary primers to amplify random DNA sequences in the genome. To identify the promising primers for RAPD analysis, 70 decamer primers of kit A, B, C and D were screened using the DNA of hybrid H-2. Based on the performance in DNA amplification, eight decamer primers were identified for RAPD analysis. Primers that produced highest number of polymorphic bands which were intense and reproducible were selected. They were OPB-07, OPB-15, OPB-l7, OPC-04, OPC-05, OPC-08, OPC-15 and OPD-02. a total of 57 scorable bands (average of 7.125 bands per primer) were generated by the selected eight primers of which six were monomorphic and the remaining 51 were polymorphic (89.47%). The estimation of Jaccard’s coefficients and construction of dendrogram by using UPGMA revealed the presence and extent of genetic similarities among the 20 monopodial orchid hybrids. The overall similarity coefficients ranged from 0.40 to 0.84. Cluster analysis revealed that at 0.69 similarity coefficient, the 20 monopodial orchid hybrids got divided into six groups. Among the 20 hybrids, H-18, H-7, H-13, H-17, H-19 and H-20 stood separately in clusters II, III A, III B, IV, V and cluster VI respectively. This substantiates the moderately broad distribution of genetic variability, which can be attributed to the broad genetic base in their ancestry. Cluster I A contained four hybrids viz., H-1, H-10, H-3 and H-9. This grouping is justified by the presence of a common parent i.e., Arachnis Maggie Oei Red Ribbon in their parentage. Moreover, two common species viz., Arachnis hookeriana and Arachnis flos-aeris are involved in their ancestry. All these support their belonging to the same cluster. The three hybrids such as H-5, H-6 and H-8 fell in cluster I B. RAPD technique is relatively simpler, quicker, less expensive and non-radioactive than other molecular characterization techniques. The results of present investigation proved that it can detect sufficient polymorphisms in genetic distance studies in monopodial orchids.ThesisItem Open Access Village level livestock and poultry production under the industrialization scenario(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2007) Rajaganapathy, V; KAU; Francis, XavierAn in depth assessment and analysis of two villages, one an industrial area and the other an agrarian hamlet, was taken up. The effect of industrialization and pollution in village level livestock and poultry production and toxic heavy metals content as an indicator of pollution in water, soil, fodder and in biological samples were studied. Based on the observation and scientific validations, changes in livestock farming system and methodologies to alleviate the extent of pollution were sorted out. This study was conducted in Plachimada village - industrial area - in Perumatty Panchayat and in Nallepilly village- control area in Nallepilly Panchayat in Chittoor taluk in Palakkad district. A detailed survey of the existing husbandry systems was done in the selected study area using a schedule. Collection of samples of water, soil, fodder and biological samples milk, meat, egg, blood and dung samples were collected and examined from both industrial and control area. The samples were analyzed for presence of heavy metals like copper, cadmium, lead and arsenic by using Atomic absorption spectrophotometer. A detailed survey was done among fifty farmers from the Plachimada (industrial area) and Nallepilly (control area) villages respectively. The farmers were interviewed with a schedule and the data was obtained. The socio-economic status of the farmers indicated that the middle aged persons were more involved in the agricultural and animal husbandry activities. Most of the farmers had an average monthly income per family of Rs. 2000 in the study area. Cultivation of crops and plantations were less in both industrial and control area. The study revealed that majority of the farmers possessed dairy cattle with major source of income from livestock farming in both these study areas. Economic viability of rearing cattle was better in control area than that of industrial area. Most of the farmers surveyed stayed very near to the industry in the industrial zone. The study revealed that nearness of the industry to the grazing lands may be affecting their livestock farming system. The presence of heavy metals cadmium and lead in drinking water and soil samples of industrial area was more than that of the control zone and the WHO (1995) recommendations. The presence of copper and cadmium in meat samples was higher whereas cadmium and lead in blood samples of the industrial area was found more than that of the control zone and the permitted levels as per the WHO recommendations. The level of copper, cadmium and lead in fodder, milk, egg and dung samples of the industrial area was found more than that of the control zone and the permitted levels as per the WHO recommendations. This study aimed also to find out the relationship between water, soil, plant and animal system. Correlation of fodder and blood heavy metals with other biological samples was done in industrial area. Fodder lead showed a highly significant correlation with milk lead content. Correlation between fodder arsenic and milk arsenic concentration was also significant. Correlation between fodder cadmium with blood cadmium was significant. Correlation was observed between fodder lead with dung lead values. Correlation existed between blood copper with dung copper at a significant level. Similarly correlation of fodder and blood heavy metals with other biological samples was done in control area also. Blood and fodder cadmium was significant and positively correlated. Correlation of fodder lead with milk and dung lead were significant. Fodder arsenic and milk arsenic were significantly correlated. In blood the copper content of milk and dung showed a positive significant correlation. The heavy metals lead, cadmium, arsenic and mercury from industrial pollution are of high environmental concern due to their toxicity even at low concentrations. These metals may persist in the system for several days due to cumulative nature and may cause severe health effects in man and animals. Bioaccumulation of toxic heavy metals, especially lead, cadmium and arsenic in milk, meat and in egg have evoked great concern during the recent years. Heavy metals contaminants may enter the animal system through pollution of air, water, soil, feed polluted by industrial sources. From the present study it was found that the industrial area had contaminated water, soil, and fodder under observation. The heavy metal contamination was from copper, cadmium and lead whereas arsenic was almost within the permissible limits. The biological samples milk, meat, egg, blood and dung collected from the industrial zone were also contaminated with copper, cadmium and lead when compared with the control area samples. The level of heavy metals in the biological samples of industrial area was above the WHO permissible limits. Arsenic was present within the maximum permissible level in all the biological samples. It is also concluded that industrialization may add pollutants to the area thereby causing an indirect effect on livestock production systems and in turn to the human beings. The following recommendations may be suggested. 1. Monitoring the level of pollution in water, soil, plants and fodder crops, animals and in man in industrial zones at regular intervals. 2. Treatment of waste water and other discharges/effluents from industries with implementation of strict rules and regulations 3. Safe disposal of the discharges, effluents and waste materials from the industries and factories. Efficient waste management to be adopted. 4. To create awareness among farming community to adopt better management practices. 5. Encouragement of organic farming. 6. Recommendation of rearing indigenous animals and adoption of Mixed farming - system to improve economic status of farmers. 7. Phytoremediation may be done to decontaminate soil and water to reduce the soil heavy metal content. 8. Identify and growing of trees/ plants to reduce the level of toxic elements from the industrial zones.ThesisItem Open Access Concurrent growing of green manure crops in dry and wet seeded rice(Department of Agronomy, College of Horticulture, Vellanikkara, 2008) Anitha, S; KAU; Jose, MathewAn investigation entitled ‘Concurrent growing of green manure crops in dry and wet seeded rice’ was conducted at Agricultural Research Station, Mannuthy to develop a comprehensive technology package for the system of concurrent growing of green manure crops in direct seeded rice. The investigation consisted of four experiments viz., Experiment Ia - Concurrent growing of green manure crops in dry seeded rice, Experiment Ib - Residual effect of concurrent growing of green manure crops in dry seeded rice, Experiment IIa - Concurrent growing of daincha in wet seeded rice and Experiment IIb - Residual effect of concurrent growing of daincha in wet seeded rice. The experiment on concurrent growing of green manure crops in dry seeded rice consisted of two green manure crops (horse gram and cowpea), three methods of incorporation (Incorporation by cono weeder, desiccation by 2,4 - D spray @1.0 kg ha-1 and allowing for self decomposition) and two levels of nitrogen (100 and 75% of recommended N) and a control without green manure crops . Among the two green manure crops, cowpea was the best in supplying the required quantity of green matter with 25 per cent savings of N fertilizer and a substantial reduction in weed incidence. All the three methods of incorporation were found to be equally effective. Hence in places where there is a difficulty in self decomposition, cowpea can be effectively incorporated by conoweeder or by spraying 2, 4-D. The cowpea variety used in this study was a short duration bush type variety facilitating additional pod yield from cowpea before it was incorporated. Concurrent growing of cowpea along with dry seeded rice is a viable system as it resulted in increased productivity, profitability and sustainability dry seeded rice. In the experiment to study the residual effect of concurrent growing of green manure crops in dry seeded rice, the main plots of dry seeded rice crop during the 1st crop season were divided into two sub plots and applied 100 and 75 per cent N to the subplots. The results revealed that concurrent growing of green manure crops were effective in increasing the yield and sustaining soil fertility status compared to pure crop of rice receiving 5 t ha-1 of FYM and 100 per cent of the recommended nitrogen. But there was no savings of N fertilizer to the succeeding transplanted rice crop as the highest yield of succeeding rice was achieved only when it received 100 per cent of the recommended N. The profitability of the rice- rice cropping system was the highest with concurrent growing of cowpea in dry seeded rice. The experiment on concurrent growing of daincha in wet seeded rice consisted of two stages of incorporation of daincha (20 DAS and 30DAS), three methods of incorporation (Incorporation by cono weeder, desiccation by 2, 4 - D spray @1.0kg ha-1 and Desiccation by Chlorimuron ethyl+ met sulfuron methyl spray @ 4.0 g ha-1 met sulfuron methyl spray @ 5 g ha-1) and two levels of nitrogen (100 and 75% of recommended N) and a control without daincha. Results indicated that concurrent growing of daincha can be successfully practiced in wet seeded rice with improved productivity. Incorporation of 30 days old daincha employing any of the three methods of incorporation was equally effective in increasing the productivity and profitability of wet seeded rice. Incorporation of daincha at 30 DAS could add about 14 t ha-1 of green matter with minimum investment and resulted in 70 per cent weed suppression. Concurrent growing of daincha receiving both 100 and 75 per cent of recommended N produced significantly higher yield than pure crop of rice receiving 5 t ha-1 of FYM and 100 per cent of the recommended nitrogen. Hence there was a saving of 25 per cent N without affecting the yield. The highest yield was obtained when 100 per cent of recommended N was applied to the system. The treatments for the experiment to study the residual effect of concurrent growing of daincha in wet seeded rice were similar to that of dry seeded rice. The results showed that concurrent growing of daincha and its incorporation at 30 DAS was effective in increasing the yield of succeeding rice and sustaining soil fertility status compared to pure crop of rice receiving 5 t ha-1 of FYM and 100 per cent of the recommended nitrogen. The highest yield of succeeding rice was achieved only when it received 100 per cent of the recommended N hence there was no savings of N fertilizer to the succeeding transplanted rice crop. Concurrent growing of daincha in wet seeded rice increased the profitability of the rice- rice cropping system. Concurrent growing of green manure crops in dry and wet seeded rice is a management alternative to reduce the production cost and to increase the yield of rice and is a practical model for sustainable rice production.ThesisItem Open Access Comparative efficacy of bromocriptine, cabergoline and thyroxine in inducine oestrus in bitches(Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, 2008) Ajitkumar, G; KAU; Sreekumaran, TDetailed survey on breeding, feeding and management of 1721 dogs belonging to 817 owners was conducted in different parts of Kerala State. Among the dogs surveyed, 518 (30.10%) were male and 1203 (69.90%) were female. Breed-wise details of dogs maintained by individual owners revealed German shepherd dog (20.74%) as the most popular breed of dog followed by Labrador retriever (18.13%), Dachshund (11.39%), Rottweiler (9.12%) and Spitz (5.52%). The percentage of local non-descript dogs was found to be 14.64 only. The prevalence, nature and magnitude of anoestrus among the female dogs were evaluated based on breeding details. Haematological, hormonal, mineral and cholesterol profile of anoestrous bitches were estimated. Data on breeding revealed that 92.93 per cent of bitches were allowed to breed and the remaining 7.07 per cent were kept as virgin throughout their lifetime. The age at first breeding in majority of the bitches (57.85%) was between 1 to 2 years with an average of 16.30 months. The average number of oestrus periods and the number of breeding per year were 2.14 ± 0.24 and 1.47 ± 0.32 respectively. The average duration of proestrual bleeding was found to be 10.87 ± 1.24 days. Most of the bitches (51.97 %) were bred twice in one oestrus and the period of acceptance varied from 4 to 22 days with an average of 8.94 ± 2.18 days. The incidence of failure of conception, abortion, stillbirth and pseudopregnancy were 18.87, 5.72, 15.56 and 13.69 per cent respectively. The neonatal problems encountered in 13.88 per cent of bitches included agalactia / hypogalactia / mammitis (5.29%), cannibalism (2.65%), puppy fading syndrome (2.09%), puerperal tetany (1.98%) and rejection of young one by dam / poor mothering ability (1.87%). Among the bitches surveyed, 134 (11.14%) were identified as in the stage of anoestrus, 65.67 per cent in primary and 34.33 per cent in secondary anoestrus and the average age was 22.68 and 34.34 months respectively. The average duration of secondary anoestrus was 11.71 months. Analysis of breed-wise prevalence revealed that it was high in breeds such as Dachshund (18.18%), Labrador retriever (16.74%), Great Dane (16.67%), Dalmatian (15.38%) and Doberman pinscher (14.00%) and was lowest (2.44%) among local non-descript bitches. Data on body condition revealed that 42 per cent of the anoestrous bitches were with the ideal body score of five, 46 per cent with body score more than five (obese) and 12 per cent with body score less than five (lean). Analysis of haematological profile of anoestrous bitches revealed normochromic normocytic anaemia. The mean serum progesterone, prolactin and thyroxine levels in anoestrous bitches were of 0.57 ± 0.03 ng/ml, 0.75 ± 0.05 ng/ml and 1.80 ± 0.06 µg/dl respectively. The mean serum calcium and phosphorus levels in anoestrous bitches were 8.94 ± 0.24 and 3.48 ± 0.12 mg per cent respectively. The mean serum iron, copper, cobalt, zinc, manganese and cholesterol levels were found to be within the normal range. Oestrus induction trials were carried out in forty anoestrous bitches and the data obtained were compared with that of control bitches. Out of 10 animals treated in each group, five (50%) in Group I (bromocriptine @ 50 μg/kg. body weight), nine (90%) in Group II (cabergoline @ 5 μg/kg. body weight), eight (80%) in Group III (thyroxine @10 μg/kg. body weight) and seven (70%) in Group IV (thyroxine @ 5 μg/kg. body weight) evinced proestrual bleeding. The mean treatment onset to proestrus in Groups I, II, III and IV was 28 ± 3.39, 13.44 ± 3.12, 24.50 ± 3.18 and 33 ± 2.21 days respectively. The average duration of proestrus in the treatment groups was 9.80 ± 0.86, 10.11 ± 0.68, 11.25 ± 0.88 and 10.71 ± 0.68 days respectively as against 9.70 ± 0.42 days in the control group. The average duration of oestrus in the four treatment groups and the control group was 7.60 ± 0.24, 8 ± 0.29, 8.5 ± 0.63, 7.85 ± 0.46 and 8 ± 0.39 days respectively. Fifty per cent of the animals treated with bromocriptine and 10 per cent of the animals treated with cabergoline exhibited nausea and vomiting as side effects, whereas none of the animals treated with thyroxine exhibited side effects. The mean serum progesterone and prolactin levels during anoestrus, on the first day of proestrus and on the day of first breeding did not differ significantly within and between the treatment groups and the control group. The mean serum thyroxine level during anoestrus and on the first day of proestrus did not differ significantly within and between the treatment and the control groups, whereas on the day of first breeding the mean thyroxine level recorded statistically significant difference between the treatment and control groups, the lowest being 1.56 ± 0.18 μg/dl in Group I and the highest being 2.10 ± 0.13 μg/dl in Group IV. Animals treated with levothyroxine (Group III & IV) exhibited a slight increase in the mean level of serum thyroxine on the first day of bleeding as well as on the day of first breeding, but the values were found to be within the normal limits. The haemoglobin level and total erythrocyte count were found to be increased on the first day of proestrus and on the day of first breeding in animals of Group III and IV. The mean serum phosphorus level on the first day of proestrus and on the day of first breeding in the treatment groups were found to continue at low levels as in the anoestrous stage. The mean serum copper level on the first day of proestrus exhibited statistically significant difference between the treatment and control groups. The mean serum level of calcium, iron, cobalt, zinc and manganese during anoestrus, on the first day of proestrus and on the day of first breeding did not reveal statistically significant difference between groups. The overall conception rate in the treatment groups were 40, 70, 50 and 40 per cent respectively as against 70 per cent in the control group. The average gestation length and litter size did not differ significantly between the treatment and control groups.
