Thumbnail Image

Birsa Agricultural University, Ranchi

Browse

20181
Reset filters
Author: BAKHLA, RASHMI × End date: 2018 × Start date: 2018 × Type: Thesis × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Genetic divergence and traits association studies in Rauvolfia serpentina (L.) Benth. Ex Kurz (Sarpgandha)
    (Birsa Agricultural University, Ranchi, Jharkhand-6, 2018) BAKHLA, RASHMI; Kumar, Jai
    Keeping in view the importance of divergence study, a systematic research trial was undertaken to quantify extent of divergence of different Sarpgandha germplasm through its qualitative and quantitative traits at AICRP (M&APs) farm, BAU, Ranchi with the following objectives. 1. To estimate the extent of genetic divergence and clustering pattern among selected germplasm of Sarpgandha 2. To screen out superior germplasm of Sarpgandha on the basis of quantitative traits 3. To find out percentage contribution of quantitative traits towards its genetic divergence 4. To study trait association pattern with regards to yield to facilitate selection of traits 5. To determine heritability and genetic advance of quantitative traits useful for improvement. Research was laid out in RBD with 25 treatments replicated thrice. Experimental material consisted of 25 germplasm of Sarpgandha (BRS1 to BRS25) collected from different districts of Jharkhand. Different qualitative and quantitative parameters were measured as per NBPGR guidelines. The replicated data of all the 25 germplasm for quantitative traits was subjected to genetic divergence analysis, analysis of variance, correlation matrix, path value analysis and principal component analysis. 15 germplasm of Sarpgandha showed semi-erect plant growth habit, while 10 had erect. Three types of leaf arrangement were found namely verticilite (16), alternate (5) and opposite (4). The root colour of 17 germplasm was pale brown while 8 germplasm had grayish brown root colour. Two type of inflorescence were found namely corymbose (14) and umbellate (11). Flower colour of 11 germplasm was pink and rest 14 germplasm were white. The fruit shape of six germplasm was globose, 9 were ovoid and 10 were sub-globose. Two type of fruit colour were noticed, and out of 25 germplasm, 19 had dark purple colour and six had brownish colour. Seed shape of 13 germplasm was elliptical and 12 were obovate. Significant differences were noticed in the plant height and maximum value was recorded for BRS2 (67.66cm) and minimum by BRS20 (37.33). Significant differences were noticed in stem diameter and maximum value was noticed for BRS15 (11.63mm), and minimum for BRS4 (7.30mm). In case of number of primary branches/plant, maximum value was noticed for BRS1 (7.00), and minimum for BRS7 (2.33). Significant differences were observed for number of leaves/plant and its maximum value was recorded for BRS23 (69.33) and least by BRS20 (24.33). Significant difference was also observed for number of nodes/plant and maximum value was recorded for BRS2 (13.66) and minimum for BRS12 (7.66). Significant differences were observed in case of length of inter-nodes and its maximum value for recorded BRS8 (10.85cm) and minimum in BRS7 (6.42cm). Maximum crown spread in N-S and E-W directions was recorded for BRS7 (33cm and 30.66cm) respectively and its minimum value observed for BRS25 (19.16cm and 20.16cm) respectively. Significant differences were also observed in case of inflorescence length and maximum value observed for BRS16 (10.31cm) and minimum value BRS21 (6.24cm). Maximum leaf length was recorded for BRS25 (13.17cm) which varied significantly from its minimum value recorded for BRS6 (9.50cm). In case of leaf width significant difference was observed with its maximum value for BRS10 (4.65 cm) and minimum for BRS7 (3.06 cm). Significant difference was observed in the number of inflorescence/plant and its maximum value was recorded for BRS2 (13.66) and minimum for BRS6 (2.33). Number of flower/inflorescence was also varied significantly and its maximum value was recorded for BRS18 (94.66) and minimum for BRS7 (17.66). In case of number of fruits/inflorescence, significant differences were observed with its maximum value BRS18 (88.66) and minimum for BRS7 (12.66). Number of seeds/fruit was also varied significantly with its maximum value BRS2 (1.72) and minimum for BRS11 and BRS22 (1.05). Maximum root length was recorded for BRS2 (88.16 cm) which varied significantly with its minimum value of BRS20 (68.03 cm). Significant differences were observed in root diameter with its maximum value in BRS4 (17.31 mm) and minimum in BRS18 (13.05 mm). As regards to dry root yield/plant, maximum value was recorded for BRS9 (83.32 g) which varied significantly with minimum value recorded for BRS20 (42.85 g). In case of seed yield/plant, maximum value recorded for BRS23 (21.89 g) which varied significantly with its minimum value recorded for BRS5 (1.51 g). Among the sources of genetic diversity in Sarpgandha germplasm, maximum percentage of contribution was shown by seed yield/plant (51.33%) followed by inflorescence length (19.33%) and number of flower/inflorescence (11.67%). Seven clusters were formed through genetic divergence analysis, out of which cluster I contains maximum 9 number of germplasm. Maximum inter cluster distance was observed between cluster III and cluster VI (13.59) followed by cluster II and cluster III (12.46). Correlation matrix of different plant characters showed plant height had highly significantly positively correlated with dry root yield/plant (0.594) and significant positive correlation between root length and dry root yield/plant (0.414). Path analysis of different growth parameter affecting seed yield/plant indicated maximum direct positive impact by no. of flowers/ inflorescence (0.658) and maximum direct negative impact by no. of fruits/inflorescence (-0.486). In case of dry root yield/plant, maximum direct positive effect was shown by plant height (0.590) and maximum direct negative effect was by root diameter (- 0.472). Maximum heritability was shown by seed yield/plant (97.34%) followed by inflorescence length (88.42%). However, maximum genetic advance was also shown by seed yield/plant (129.13%) followed by no. of fruits/inflorescence (79.59%). So to conclude, as regards to estimate the extent of genetic divergence and clustering pattern among selected germplasm of Sarpgandha, seven clusters were formed, out of which cluster I consisted of 9 germplasm, followed by cluster II (8 germplasm) and cluster III (4 germplasm). Maximum inter cluster divergence was found between cluster III and cluster VI (13.59) followed by cluster II and cluster III (12.46) and cluster III and VII (10.44). So parents may be selected for hybridization program from these clusters i.e. cluster (III & VI), (II & III) and (III & VII). The extent of genetic divergence was ranged between 3 to 30% which may be considered as low genetic divergence. As regards to screen out superior germplasm of Sarpgandha on the basis of quantitative traits, on the basis of dry root yield/plant, three germplasm namely BRS9 followed by BRS23 & BRS5 may be selected as superior germplasm because they produced maximum 83.32, 78.25 & 77.97 g dry root yield/plant respectively, which was significantly superior to rest of the germplasm. On the basis of seed yield /plant, three germplasm namely BRS23 followed by BRS21 & BRS12 may be selected as superior germplasm because they produced maximum 21.89, 21.06 & 20.79 g seed yield/plant respectively, which was significantly superior to rest of the germplasm. As regards to find out percentage contribution of quantitative traits towards its genetic divergence, maximum genetic divergence was shown by seed yield/plant (51.33 %), inflorescence length (19.33%) & number of flowers /inflorescences (11.67 %). As regards to study trait association pattern with regards to yield to facilitate selection of traits, plant height showed high significant positive correlation with dry root yield/plant (0.594) & significantly positively correlated with root length (0.414). Again maximum direct positive effect on dry root yield/plant was shown by plant height (0.590), followed by number of leaves/plant (0.224) & root length (0.135). So plant height & root length may be selected as suitable traits for improving dry root yield/plant in Sarpgandha. As regards to determine heritability and genetic advance of quantitative traits useful for improvement, maximum heritability was shown by seed yield/plant (97.34%) followed by inflorescence length (88.42%) & number of fruits/inflorescence (83.28%). Maximum genetic advance was shown by seed yield/plant (129.13%) followed by number of fruits/inflorescences (75.59%).